Abbkine full portfolio of loading control and epitope tag antibodies

With full portfolio of loading control and epitope tag antibodies, Abbkine launched series of conjugated antibodies, including HRP, Biotin, FITC, Cy3 and AbFluor™ dyes to meet the diversified demands of researchers. Also, the agarose and magnetic beads conjugated tag antibodies specifically for immunoprecipitation are now available.

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Abbkine new arrival-Annexin V Apoptosis Detection kit

Apoptosis is a form of programmed cell death to remove unwanted, damaged, or senescent cells from tissues. In normal cells, the negative phospholipids reside on the inner side of the cellular membrane while the outer surface of the membrane is occupied by uncharged phospholipids (PS). After a cell has entered apoptosis, the negatively charged PS are transported from the inner to the outer leaflet of the plasma membrane, thus exposing PS to the external cellular environment. The human anticoagulant, Annexin V, is a 35–36 kDa Ca²⁺-dependent phospholipid-binding protein that has a high affinity for PS. Annexin V labeled with a fluorophore or biotin can identify apoptotic cells by binding to PS exposed on the outer leaflet.

Propidium iodide (PI) is a membrane-impermeant DNA-binding dye that is commonly used to selectively stain dead cells in a cell population. PI is excluded by live cells and early apoptotic cells, but stains necrotic and late apoptotic cells with compromised membrane integrity. PI can be excited by the 488, 532, or 546 nm laser lines, and emits red fluorescence.

Based on Abbkine strong cell platform and advanced technology, Abbkine Annexin V Apoptosis Detection kits are generated at the right moment. Abbkine Annexin V Apoptosis Detection kit contains Annexin V labeled with Abbkine proprietary  AbFluor™ dyes, which allows the identification and quantitation of apoptotic cells by flow cytometry or fluorescence microscopy. Simultaneous staining of cells with AbFluor™ dyes and propidium iodide (PI) allows the discrimination of intact cells, early apoptotic and late apoptotic or necrotic cells.

Please see the below list for details

Product name	Cat#	Abs/Em
Annexin V-AbFluor™ 405 Apoptosis Detection kit	KTA0001	Annexin V- AbFluor™ 405: Abs/Em = 404/431 nm; PI: Abs/Em = 535/617 nm (with DNA)
Annexin V-AbFluor™ 488 Apoptosis Detection kit	KTA0002	Annexin V- AbFluor™ 488: Abs/Em = 491/517 nm; PI: Abs/Em = 535/617 nm (with DNA)
Annexin V-AbFluor™ 555 Apoptosis Detection kit	KTA0003	Annexin V- AbFluor™ 555: Abs/Em = 555/565 nm
Annexin V-AbFluor™ 647 Apoptosis Detection kit	KTA0004	Annexin V- AbFluor™ 555: Abs/Em = 555/565 nm; PI: Abs/Em = 535/617 nm (with DNA)

 

Abbkine offers a variety of Annexin V conjugates labeled with our outstanding series of AbFluor™ dyes. AbFluor™ dyes have equal or much better labeling performance than other fluorescent labeling dyes such as FITC, TRITC, Cy® dyes, Alexa Fluor® and Dylight® labeling dyes by having combined advantages in brightness, photostability, specificity and novel features ideal for in vivo imaging and flow cytometry.

Take Annexin V-AbFluor™ 488 Apoptosis Detection kit for example. The typical data is as below.

Hela cells were induced with camptothecin for 24 hours and stained with Annexin V- AbFluor™ 488 Apoptosis Detection Kit. The combination of AbFluor™ 488 and propidium iodide allows for the distinction between early apoptotic cells (Annexin V- AbFluor™ 488 positive), late apoptotic and/or necrotic cells (Annexin V- AbFluor™ 488 and propidium iodide positive), and viable cells (unstained).

 

Abbkine Scientific Co., Ltd.

Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc. Abbkine is aligned with, and responsive to, our customers’ ever-changing needs, making Abbkine a preferred discovery partner internationally.

His Tag Antibodies ||| Abbkine

Abbkine provides unconjugated and conjugated with AbFluor™ 350, 405, 488, 555, 594, 647,680, Cy3, Cy5, FITC, HRP, Agarose, Magnetic Beads antibodies for your studying in the His Tag Antibodies research area.

[caption id="attachment_84014" align="aligncenter" width="412"] Immunofluorescence staining (1:2000) of HA fusion protein in 293 cells with red and counterstained with DAPI[/caption]

More product details, please click below links:

Quick links to His Tag Antibodies
Conjugate	Catalog no	Applications	Host
unconjugated	A02050	WB, IF, IP	Mouse
AbFluor™ 350	A02050A350	IF	Mouse
AbFluor™ 405	A02050A405	IF	Mouse
AbFluor™ 488	A02050A488	IF	Mouse
AbFluor™ 555	A02050A555	IF	Mouse
AbFluor™ 594	A02050A594	IF	Mouse
AbFluor™ 647	A02050A647	IF	Mouse
AbFluor™ 680	A02050A680	IF	Mouse
Cy3	A02050CY3	IF	Mouse
Cy5	A02050CY5	IF	Mouse
FITC	A02050FIT	IF	Mouse
Agarose	A02050AGB	IP	Mouse
Magnetic Beads	A02050MGB	IP	Mouse
unconjugated	A02051	WB	Rabbit
HRP	A02051HRP	WB	Rabbit
HRP	A02055	WB	Mouse
unconjugated	ABM40129	WB	Mouse
unconjugated	ABM40141	WB	Mouse
unconjugated	ABP50077	WB, ELISA	Rabbit

About Abbkine Scientific Co., Ltd.

Abbkine Scientific Co., Ltd. is a leading biotechnology company that focuses on developing and providing innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc. Find more details, please visit the website at Abbkine.

His Tag Antibodies ||| Abbkine

Abbkine provides unconjugated and conjugated with AbFluor™ 350, 405, 488, 555, 594, 647,680, Cy3, Cy5, FITC, HRP, Agarose, Magnetic Beads antibodies for your studying in the His Tag Antibodies research area.

[caption id="attachment_84014" align="aligncenter" width="412"] Immunofluorescence staining (1:2000) of HA fusion protein in 293 cells with red and counterstained with DAPI[/caption]

More product details, please click below links:

Quick links to His Tag Antibodies
Conjugate	Catalog no	Applications	Host
unconjugated	A02050	WB, IF, IP	Mouse
AbFluor™ 350	A02050A350	IF	Mouse
AbFluor™ 405	A02050A405	IF	Mouse
AbFluor™ 488	A02050A488	IF	Mouse
AbFluor™ 555	A02050A555	IF	Mouse
AbFluor™ 594	A02050A594	IF	Mouse
AbFluor™ 647	A02050A647	IF	Mouse
AbFluor™ 680	A02050A680	IF	Mouse
Cy3	A02050CY3	IF	Mouse
Cy5	A02050CY5	IF	Mouse
FITC	A02050FIT	IF	Mouse
Agarose	A02050AGB	IP	Mouse
Magnetic Beads	A02050MGB	IP	Mouse
unconjugated	A02051	WB	Rabbit
HRP	A02051HRP	WB	Rabbit
HRP	A02055	WB	Mouse
unconjugated	ABM40129	WB	Mouse
unconjugated	ABM40141	WB	Mouse
unconjugated	ABP50077	WB, ELISA	Rabbit

About Abbkine Scientific Co., Ltd.

Abbkine Scientific Co., Ltd. is a leading biotechnology company that focuses on developing and providing innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc. Find more details, please visit the website at Abbkine.