Featured DDDDK tag antibodies

DDDDK tag is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, which allows elution under non-denaturing conditions. The DDDDK tag is likely to be located on the surface of a fusion protein because of its hydrophilic nature, and therefore is more likely to be accessible to antibodies. It not only has been used for studying proteins in living cells and for protein purifcation by affinity chromatography, but also has been used in the isolation of protein complexes with multiple subunits.

Abbkine offers a full range of anti-DDDDK tag antibodies for your choice. The antibodies not only include highly specific monoclonal and polyclonal DDDDK tag antibodies, but also contain AbFluor™ (350, 405, 488, 555, 594, 647, 680) dyes, Cy3, Cy5, FITC, agarose, magnetic beads, HRP conjugated anti-DDDDK tag antibodies.

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About Abbkine Scientific Co., Ltd.

Abbkine Scientific Co., Ltd. is a leading biotechnology company that focuses on developing and providing innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.  Find more details, please visit the website at Abbkine.

More epitope tag antibodies to be continued...

AbFluor™ 488 Annexin V Apoptosis Detection Kit from Abbkine

Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry is lost and PS translocates to the external leaflet.

Fluorochrome-labeled Annexin V can then be used to specifically target and identify apoptotic cells, which can be detected by fluorescence microscopy or flow cytometry.



Propidium iodide (PI) is a fluorescent nucleus dye, impermeant to live cells and early apoptotic cells, but stains late apoptotic, necrotic or dead cells with red fluorescence, binding tightly to the nucleic acids in the cell.

Once binding with DNA, the excitation and emission light of PI-DNA complex is 535 nm and 615 nm.



Abbkine AbFluor™ 488 Annexin V Apoptosis Detection Kit contains Annexin V labeled with Abbkine proprietary green fluorescent dye AbFluor™ 488, which allows the identification and quantitation of apoptotic cells by flow cytometry or fluorescence microscopy. Simultaneous staining of cells with AbFluor™ 488 and propidium iodide (PI) allows the discrimination of intact cells, early apoptotic and late apoptotic or necrotic cells.

[caption id="attachment_84161" align="aligncenter" width="635"] Hela cells were induced with camptothecin for 24 hours and stained with Annexin V- AbFluor™ 488 Apoptosis Detection Kit. The cell is a late stage apoptotic/necrotic cell with both Annexin V- AbFluor™ 488 and PI staining (green membrane with red fragmented nucleus).[/caption]

 

Abbkine AbFluor™ 488 dye is better than FITC, Alexa Fluor 488, Dylight 488 as it is brighter, not affected by pH, and has much better photostability.

Below is Abbkine Annexin V Apoptosis Detection Kits details:

[table id=15 /]

FAQ

1. Can Abbkine Annexin V Apoptosis Detection Kit detect apoptosis in animals other than humans?

Sure. Because annexin v is compatible with phosphatidylserine ( PS ), but PS has no difference among different species.

2. What’s the effect of pancreatin on adherent cells?

Pancreatin has some damage to the cell membrane. But when digested with low concentration pancreatin, the adherent cells are gently blown 2 to 3 times, centrifuged at 4 , 300 g, and 5 min, the damage caused by pancreatin can be controlled within 5 %. And there is no obvious influence on the experimental results when there is a control group.

3. Why can only use pancreatin without EDTA to digest adherent cells?

Annexin V is a Ca+ dependent protein, so EDTA cannot be added to prevent EDTA from sequestering Ca+, thus affecting annexin V and the results.

AbFluor™ 488 Annexin V Apoptosis Detection Kit from Abbkine

Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry is lost and PS translocates to the external leaflet.

Fluorochrome-labeled Annexin V can then be used to specifically target and identify apoptotic cells, which can be detected by fluorescence microscopy or flow cytometry.



Propidium iodide (PI) is a fluorescent nucleus dye, impermeant to live cells and early apoptotic cells, but stains late apoptotic, necrotic or dead cells with red fluorescence, binding tightly to the nucleic acids in the cell.

Once binding with DNA, the excitation and emission light of PI-DNA complex is 535 nm and 615 nm.



Abbkine AbFluor™ 488 Annexin V Apoptosis Detection Kit contains Annexin V labeled with Abbkine proprietary green fluorescent dye AbFluor™ 488, which allows the identification and quantitation of apoptotic cells by flow cytometry or fluorescence microscopy. Simultaneous staining of cells with AbFluor™ 488 and propidium iodide (PI) allows the discrimination of intact cells, early apoptotic and late apoptotic or necrotic cells.

[caption id="attachment_84161" align="aligncenter" width="635"] Hela cells were induced with camptothecin for 24 hours and stained with Annexin V- AbFluor™ 488 Apoptosis Detection Kit. The cell is a late stage apoptotic/necrotic cell with both Annexin V- AbFluor™ 488 and PI staining (green membrane with red fragmented nucleus).[/caption]

 

Abbkine AbFluor™ 488 dye is better than FITC, Alexa Fluor 488, Dylight 488 as it is brighter, not affected by pH, and has much better photostability.

Below is Abbkine Annexin V Apoptosis Detection Kits details:

[table id=15 /]

FAQ

1. Can Abbkine Annexin V Apoptosis Detection Kit detect apoptosis in animals other than humans?

Sure. Because annexin v is compatible with phosphatidylserine ( PS ), but PS has no difference among different species.

2. What’s the effect of pancreatin on adherent cells?

Pancreatin has some damage to the cell membrane. But when digested with low concentration pancreatin, the adherent cells are gently blown 2 to 3 times, centrifuged at 4 , 300 g, and 5 min, the damage caused by pancreatin can be controlled within 5 %. And there is no obvious influence on the experimental results when there is a control group.

3. Why can only use pancreatin without EDTA to digest adherent cells?

Annexin V is a Ca+ dependent protein, so EDTA cannot be added to prevent EDTA from sequestering Ca+, thus affecting annexin V and the results.

Abbkine will exhibit at Neuroscience 2018, San Diego, CA

Abbkine invites you to visit us at Neuroscience 2018 in San Diego, CA!

Abbkine top selling products and services will be displayed at the exhibition. There always be one type can flash into your eyes.

Neuroscience 2018

Date: 4, November -7, November, 2018

Booth: #3510 (Hall C)

Address: San Diego Convention Center

About: SfN's 48th annual meeting, Neuroscience 2018, is the world's largest neuroscience conference for scientists and physicians devoted to understanding the brain and nervous system. SfN invites you to its renowned venue where neuroscientists collaborate and network with peers, learn from experts, explore the newest neuroscience tools and technologies, and discover great career opportunities.

Welcome to visit our booth #3510 (Hall C).

30% off on Abbkine Antibodies and Annexin V Apoptosis Detection Kits

With full portfolio of loading control and epitope tag antibodies, Abbkine launched series of conjugated antibodies, including HRP, Biotin, FITC, Cy3 and AbFluor™ dyes to meet the diversified demands of researchers. Also, the agarose and magnetic beads conjugated tag antibodies specifically for immunoprecipitation are now available.

With Abbkine products becoming more and more popular with global researchers, Abbkine will conduct regular promotional activities to thank customers for their support.

From 1st, July, 2018 to 30th, September, 2018 Abbkine offers two attractive promotion campaigns to our valued end users.

Promotion 1:

30% discount for loading control antibody (Cat# A01XXX), tag antibody (Cat# A02XXX) and secondary antibody (Cat# A2XXXX).

Please contact service@abbkine.com or your local distributors for promotion list.

Promotion 2:

30% discount on the newly-launched Annexin V Apoptosis Detection kit.

Please check the below promotion list for details.

Product name	Cat#	Abs/Em
Annexin V-AbFluor™ 405 Apoptosis Detection kit	KTA0001	Annexin V- AbFluor™ 405: Abs/Em = 404/431 nm; PI: Abs/Em = 535/617 nm (with DNA)
Annexin V-AbFluor™ 488 Apoptosis Detection kit	KTA0002	Annexin V- AbFluor™ 488: Abs/Em = 491/517 nm; PI: Abs/Em = 535/617 nm (with DNA)
Annexin V-AbFluor™ 555 Apoptosis Detection kit	KTA0003	Annexin V- AbFluor™ 555: Abs/Em = 555/565 nm
Annexin V-AbFluor™ 647 Apoptosis Detection kit	KTA0004	Annexin V- AbFluor™ 647: Abs/Em = 650/665 nm; PI: Abs/Em = 535/617 nm (with DNA)

* This promotion is only available to end users. Abbkine reserves the right to cancel or refuse this promotion at any time.