Quantitative determination of LDH in samples, Abbkine CheKine™ Lactate Dehydrogenase (LDH) Assay Kit will offer you an easier method

Lactate dehydrogenase (LDH) is an oxidoreductase
found in nearly all living cells (animals, plants, and prokaryotes). LDH
catalyzes the conversion of lactate to pyruvate and back, as it converts NAD+
to NADH and back. A dehydrogenase is an enzyme that transfers a hydride from
one molecule to another.

Quantification of Lactate dehydrogenase has clinical
significance because serum levels of certain lactate dehydrogenase isoenzymes
reflect the pathological conditions of specific tissues. When tissue is damaged
by disease, injury, or toxic substances, lactate dehydrogenase is released into
the bloodstream. Because lactate dehydrogenase is a fairly stable enzyme, it
has been widely used to evaluate tissue, cell damage and toxicity.

Abbkine CheKine™ Lactate Dehydrogenase
(LDH) Assay Kit, more simple, convenient and sensitive

Product Name	CAT #
CheKine™ Lactate Dehydrogenase Assay Kit	KTB1110

【Principle】

provides a simple and easy colorimetric assay for measuring LDH in samples. In this colorimetric assay, LDH reduces NAD to NADH, which then interacts with a probe to produce a color (λmax = 450 nm), the LDH present in the sample is proportional to the signal obtained.

【Samples】

serum, plasma, cell culture supernatants, tissue/cell lysates, culture medium, fermentation and other biological fluids.

【Kit components】

Name	Storage temperature	Function
Assay Buffer	4ºC	maintain reaction environment
Lactate	-20ºC	LDH substrate
NAD	-20ºC	LDH substrate
WST-8	-20ºC, protect from light	coloration
Enhancer	-20ºC, protect from light	electron transport complex
LDH Standard	-20ºC	Prepare standard curve

【Procedure】

1. Working Reagent Preparation
2. Add standard and sample per
   well, then add Working Reagent to each well. Tap plate to mix. Immediately read
   optical density at 450nm (OD0).
3. Incubate for 30 min at 37°C in the dark.
   Read optical density at 450nm again (OD30).

【Standard Curve】

About Abbkine Scientific Co., Ltd.

Abbkine serves global scientists in the
field of proteomics and cytology and is committed to the innovation and
development of various scientific reagents related to proteomics and cytology,
expecting to accelerate the pace of life science research and drug discovery.
Proteomics products cover the preparation of samples (protein extraction,
purification, coupling), protein quantification, antibodies and kits for
protein detection. Cytology products involve cytokines (cell culture), cell
status detection, cell staining, organelle extraction, cell metabolism and
cytopathology reagents (kits). Abbkine relies on the product portfolio and
unique marketing support as the main market strategy and product innovation
mode, with ultimate aim to facilitate your research career.

CheKine™ Xanthine Oxidase Assay Kit, a great tool for study of hyperuricemia

Xanthine oxidase is a form of xanthine oxidoreductase, a type of enzyme that generates reactive oxygen species. These enzymes catalyze the oxidation of hypoxanthine to xanthine and can further catalyze the oxidation of xanthine to uric acid. These enzymes play an important role in the catabolism of purines in some species, including humans.

Xanthine oxidase (XO, EC 1.17.3.2 ) is present in appreciable amounts in the liver and jejunum in healthy individuals. Most of the protein in the liver exists in a form with xanthine dehydrogenase activity, but it can be converted to xanthine oxidase by reversible sulfhydryl oxidation or by irreversible proteolytic modification. In various liver disorders, XO is released into circulation. Therefore, determination of serum XO level serves as a sensitive indicator of acute liver damage such as jaundice.

Abbkine CheKine™ Xanthine Oxidase Assay Kit provides a simple and easy colorimetric assay for the quantitative determination of the XO present in a variety of samples. In the assay, XO oxidizes xanthine to superoxide (O2 - ). Superoxide (O2 - ) reacts with a tetrazolium salt WST-8 dye to form a water-soluble colored formazan product, which can be easily quantified colorimetrically at OD 450 nm. Therefore, the Xanthine Oxidase present in the sample is proportional to the signal obtained.

Product Name	CAT #	Detection Range
CheKine™ Xanthine Oxidase Assay Kit	KTB1070	15.6 -500 mU/mL

【Kit components】

•
Assay Buffer

•
Sample Diluent

•
WST-8

•
Enhancer

•
Xanthine Oxidase Standard (10 U/mL)

•
Xanthine

【Procedure】

1. Prepare
   the Working Reagent.
2. Add
   standard and sample per well. Then add Working Reagent per well quickly. Tap
   plate to mix briefly and thoroughly. Immediately read
   optical density at 450nm (OD0).
3. Incubate for 30 min at room
   temperature (25°C) in the dark. Read optical density at 450nm again (OD30).

【Standard Curve】

About Abbkine Scientific Co., Ltd.

Abbkine serves global scientists in the field of
proteomics and cytology and is committed to the innovation and development of
various scientific reagents related to proteomics and cytology, expecting to
accelerate the pace of life science research and drug discovery. Proteomics
products cover the preparation of samples (protein extraction, purification,
coupling), protein quantification, antibodies and kits for protein detection.
Cytology products involve cytokines (cell culture), cell status detection, cell
staining, organelle extraction, cell metabolism and cytopathology reagents
(kits). Abbkine relies on the product portfolio and unique marketing support as
the main market strategy and product innovation mode, with ultimate aim to
facilitate your research career.

New biosensor weapons, CheKine™ Glucose Oxidase Activity (GOD)Assay Kit

Glucose oxidase is a an oxido-reductase
that catalyses the oxidation of glucose to hydrogen peroxide and
D-glucono-δ-lactone. This enzyme is produced by certain species of fungi and
insects and displays antibacterial activity when oxygen and glucose are
present. Glucose oxidase is widely used to measure glucose in body fluids, as
well as residual glucose and oxygen from beverages, foods and other
agricultural products. In addition, glucose oxidase is commonly used as
biosensors to detect glucose.

Test the activity of glucose oxidase
(GOD), recommend the Abbkine CheKine™ glucose oxidase (GOD) activity kit for
you, details are as follows:

Product Name	CAT #	Detection Range
CheKine™ Glucose Oxidase Activity (GOD)Assay Kit	KTB1310	0.05 - 2 U/L

【Principle】

In this assay, GOD activity
is determined by a coupled enzyme assay, in which GOD oxidizes D-glucose
resulting in the production of hydrogen peroxide (H2O2) that reacts with
chromogen in an acid condition, produce a product that can be measured at OD
580 nm. Therefore, the glucose oxidase activity present in the sample is
proportional to the signal obtained.

【Sample】

Serum, Plasma, Cells, Tissues, Urine, Food,
Medium, other body fluids.

【Kit components】

• Assay Buffer (20x)
• Glucose (0.2 M)
• H2O2 Standard (0.88 M)
• Chromogen
• Glucose Oxidase (control)

【Simple procedure】

1. Prepare standards and sample, add Glucose
   to each tube, mix and incubate for 20 min at 37°C.
2. Transfer the mixture into a clear bottom
   96-well plate, add Chromogen per well, mix and read optical density at 580nm(OD0).
   Incubate for 10 min at 37°C in the dark. Read optical density at 580nm again(OD10).

【Standard Curve】

About Abbkine Scientific Co., Ltd.

Abbkine serves global scientists in the
field of proteomics and cytology and is committed to the innovation and
development of various scientific reagents related to proteomics and cytology,
expecting to accelerate the pace of life science research and drug discovery.
Proteomics products cover the preparation of samples (protein extraction,
purification, coupling), protein quantification, antibodies and kits for
protein detection. Cytology products involve cytokines (cell culture), cell
status detection, cell staining, organelle extraction, cell metabolism and
cytopathology reagents (kits). Abbkine relies on the product portfolio and
unique marketing support as the main market strategy and product innovation mode,
with ultimate aim to facilitate your research career.

CheKine™ Lactate Assay Kit, fast quantitative determination of L (+)-Lactate in various samples

Lactate
(CH3CH(OH)COO-) is a metabolic compound formed in animals by the action of the enzyme
lactate dehydrogenase. Lactate is produced in proliferating cells during
anaerobic conditions such as exercise. Abnormally high concentrations of
lactate have been related to pathological conditions such as cancer, diabetes,
and lactate acidosis.

L(+)-Lactate is the major lactate stereoisomer formed in human intermediary metabolism and is present in blood at levels of around 1-2 mmol/L. D(-)-Lactate can also be found in blood but only at about 1-5% of the concentration of L(+)-Lactate.

Lactic
acid is the end product of glycolysis in the body, which can be produced when
tissue hypoxia or pyruvic acid is not oxidized in time. Usually, the lactic
acid obtained from metabolism does not affect the acid-base balance in the
patient's body. However, when the violent exercise reaches a high metabolic
state or shock, the anaerobic metabolism in the tissue increases significantly,
accelerating the production of lactic acid. The body's ability to remove lactic
acid is gradually reduced, eventually leading to hyperlactacidemia, even lactic
acidosis. At this time, the blood lactate level test results showed a
significant increase. So the  lactate
level can be used as an important indicator to reflect the balance of oxygen supply
and demand in critically ill patients.

Want a quick, quantitative determination of lactic acid? Abbkine CheKine™ Lactate assay kit will give you a good experience!

Abbkine CheKine™ Lactate Assay Kit provides a convenient means for detecting L-(+)-Lactate in biological samples such as in serum or plasma, cells, culture and fermentation media. In this kit lactate is oxidized by lactate dehydrogenase to generate a product which interacts with a tetrazolium salt WST-8 dye to form a colorimetric (450 nm) product, proportional to the lactate present.

Product Name	CAT #	Detection Range
CheKine™ Lactate Assay Kit	KTB1100	Detection Range

【Kit components】

• Lactate
Assay Buffer

• Lactate
Dehydrogenase

• Lactate
Dehydrogenase Cofactor

•
WST-8

• Enhancer

•
L(+)-Lactate Standard (100 mM)

【Procedure】

1. Prepare
   the Working Reagent.
2. Add
   diluted standard and sample per well, then add Working Reagent. Tap plate
   to mix. Immediately read optical density at 450nm (OD0).
3. Incubate
   for 30 min at 37°C in the dark. Read optical density at 450nm again (OD30).

【Standard Curve】

About Abbkine Scientific Co., Ltd.

Abbkine serves global scientists in the field of proteomics and cytology and is committed to the innovation and development of various scientific reagents related to proteomics and cytology, expecting to accelerate the pace of life science research and drug discovery. Proteomics products cover the preparation of samples (protein extraction, purification, coupling), protein quantification, antibodies and kits for protein detection. Cytology products involve cytokines (cell culture), cell status detection, cell staining, organelle extraction, cell metabolism and cytopathology reagents (kits). Abbkine relies on the product portfolio and unique marketing support as the main market strategy and product innovation mode, with ultimate aim to facilitate your research career.