HMG-1 Polyclonal Antibody Review

HMGB1 (high-mobility group box 1 protein), also known as HMG1 or Amphoterin, is an intracellular protein that is regulated by multiple posttranslational modifications including acetylation, phosphorylation, and methylation. HMGB1 is released upon cell necrosis, apoptosis, and pyroptosis. It acts as a multifunctional alarmin that stimulates inflammation upon sterile or infectious insult. Receptors for HMGB1 include TLR2, TLR4, TLR9, Syndecan-3, Siglec-10, Integrin alpha M beta 2, CXCR4, TIM-3, and RAGE. It is implicated in the pathogenesis of a broad range of diseases including atherosclerosis, sepsis, cancer, neurodegenerative diseases, and autoimmunity.

HMG-1 Polyclonal Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This antibody has been tested with WB, IHC and ELISA. And Abbkine suggested starting dilutions are as follows: WB: 1:500-1:2000, IHC-p: 1:100-1:300, ELISA: 1:20000. Not yet tested in other applications.

HMGB1 encodes high mobility group box 1 that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. It applies to WB, IHC and ELISA experiments. It's convenient to choose this product.

Cleaved-Notch 1 (V1754) Polyclonal Antibody Released

Notch homolog 1, translocation-associated (Drosophila), also known as NOTCH1, is a human gene encoding a single-pass transmembrane receptor. This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch 1, when activated before birth, induces radial gliadifferentiation, but postnatally induces the differentiation into astrocytes. One study shows that Notch-1 cascade is activated by Reelin in an unidentified way. Reelin and Notch1 cooperate in the development of the dentate gyrus, according to another.

Cleaved-Notch 1 (V1754) Polyclonal Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using synthesized peptide derived from the internal region of human Notch 1. at AA rangle: 1710-1790. The reactivity of this antibody are Human, Mouse and Rat. Test confirmed the antibody can be applied in ELISA, IHC-p and WB. Abbkine suggested the starting dilutions are as follows: WB: 1:500-1:2000, IHC-p: 1:100-1:300 and ELISA: 1:20000.

Western Blot analysis of KB cells using Cleaved-Notch 1 (V1754) Polyclonal Antibody diluted at 1：500 has been shown in the left picture. Supplied as liquid solution, the  antibody is stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Glut4 Polyclonal Antibody Review

Glucose Transporter 4 (GLUT4/SLC2A4) is a member of a family of proteins that facilitate glucose transport. In response to insulin GLUT4 translocates from low density microsomes to the plasma membrane. Activation of phosphatidylinositol-3-OH kinase is required for this trafficking event but is not sufficient. Insulin resistant subjects with and without diabetes show abnormal accumulation of GLUT4 in a dense membrane compartment in muscle and adipocytes from which insulin is unable to recruit GLUT4 to the cell surface.

Glut4 Polyclonal Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This antibody has been tested with WB and ELISA. And Abbkine suggested starting dilutions are as follows: WB: 1:500-1:2000, ELISA: 1:20000. Not yet tested in other applications.

I am happy to choose this product. The pictures are beautiful! It is responsible to say that Glut4 Polyclonal Antibody has a reliable quality. It is worth recommending.

Cleaved-Caspase-9 (D353) Polyclonal Antibody Released

Caspase-9, which is activated by association with the Apaf-1 (apoptotic protease-activating factor-1) apoptosome complex, cleaves and activates the downstream effector caspases-3 and -7, thereby executing the caspase-cascade and cell-death programme. Although caspase-9 does not need to be cleaved to be active, apoptotic cell death is always accompanied by autocatalytic cleavage and by further downstream effector caspase-dependent cleavage of caspase-9. Caspase 9 is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.

Abbkine Cleaved-Caspase-9 (D353) Polyclonal Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using synthesized peptide derived from the C-terminal region of human Caspase-9 at AA rangle: 280-360. The antibody is react with mouse and rat and the verified applications are WB, IHC-P and ELISA. Abbkine suggested the starting dilutions are WB: 1:500-1:2000, IHC-p: 1:100-1:300, ELISA: 1:20000.

Supplied as liquid , the concentration of the Cleaved-Caspase-9 (D353) antibody is 1mg/ml. Uniprot accession of the relative protein of the antibody is P55211, and gene ID is 842. The antibody is stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

AR Polyclonal Antibody Review

AR (androgen receptor) is a member of the steroid superfamily of ligand-dependent transcription factors. They regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. AR consists of an amino terminal modulating domain, a central DNA-binding domain, a hinge region and a carboxyl terminal ligand binding domain. Defects in AR are the cause of androgen insensibility syndrome (genital disorders), X-linked spinal and bulbar muscular atrophy (Kennedy disease), metastatic prostate cancer and infertility male syndrome.

AR Polyclonal Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This antibody has been tested with WB and ELISA. And Abbkine suggested starting dilutions are as follows: WB: 1:500-1:2000, ELISA: 1:10000. Not yet tested in other applications.

The expression of AR is reportedly inversely correlated with histologic grade i.e. well differentiated prostate tumors show higher expression than the poorly differentiated tumors. AR Polyclonal Antibody worked very well. I am satisfied with the results of the experiment.

Next Generation Sequencing

Empowered to ultimately decipher the blueprint of life, Next Generation Sequencing (NGS) is the answer to how we can harness the potential locked in our genetic material. A successful culmination of cutting edge R&D, NGS has come to represent a monumental technological advancement that is not only poised to redefine many aspects of scientific research, but also pave the way for the emerging field of personalized medicine.



The Abbkine platform for Next Generation Sequencing has been created at Wuhan Institute of Biotechnology, the Illumina Certified Service Provider in Biolake Wuhan. An Illumina Hi-Seq 2500 sequencer has been purchased and is dedicated exclusively to the project. Abbkine's premium NGS services will not only save valuable time and money, but also provide the most reliable and comprehensive solution for the project's needs.

Committed to making newer technologies attainable to modern researchers, Abbkine offers a wide range of sequencing services on the advanced Illumina® sequencing platforms at accessible prices and rapid turnaround times.

Abbkine's Next Generation Sequencing (NGS) service is a one-stop full service: Understanding our client’s specific needs, producing and delivering the products according to schedule, and providing technical support post-delivery. We respect our clients’ confidential information, and guarantee that all intellectual property remains with the client. Kindly email us with your antibody production demands and quote request at service@abbkine.com.

Cleaved-Caspase-8 (D384) Polyclonal Antibody Released

Apoptosis is a physiological process of cellular autodestruction. The central component of this machinery is a proteolytic system involving a family of proteases called caspases. Fas(APO-1/CD95) is a transmembrane receptor protein which induces apoptosis upon activation. In apoptosis triggered by Fas, a subset of caspases is activated. Among them, caspases-8 (FLICE/MACH) is the first step in the cas2 cade of apoptotic events induced by Fas. Cleavaged caspase-8 then activates other downstream caspases including caspase-9, thereby commiting the cell to undergo apoptosis. Many studies show that caspase-8 is related to the pathogenesis of certain diseases and injuries such as cancers, neurodegenerative disease, parasitosis, trauma and so on in human beings.

Abbkine Cleaved-Caspase-8 (D384) Polyclonal Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using synthesized peptide derived from the C-terminal region of human Caspase-8. at AA rangle: 310-390. The antibody is react with human and the verified applications are WB and ELISA. Abbkine suggested the starting dilutions are  WB: 1:500-1:2000, ELISA: 1:40000.

Supplied as liquid , the concentration of the Cleaved-Caspase-8 (D384) antibody is 1mg/ml. Uniprot accession of the relative protein of the antibody is Q14790, and gene ID is 841. The antibody is stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.