Abbkine Scientific has recently announced the official release of its new product, the EliKine™ Human TGF-β1 ELISA Kit. This is part of the company’s commitment to ensuring an easier and more effective research process. Otherwise known as the Human TGFB1 ELISA Kit, the product is coming as an addition to the long list of research products and solutions from the scientific research giant.
Gene TGF-β is also known as LAP, so the EliKine™ Human TGF-β1 ELISA Kit is often called EliKine™ Human LAP ELISA Kit, the product is one of EliKine™ ELISA Kits, the featured Kit is probably the first of its kind in the industry with features and benefits that distinguish it from other such products on the market. Over the years, scientists, researchers and investigators alike have had to deal with products that are either exorbitantly priced or those that fail to deliver on their claims.
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The recent launch of the research kit by Abbkine Scientific therefore signals a new beginning in the scientific research world, with the major benefit being its high sensitivity and excellent specificity for detection of Human TGF-β1.
In addition to the benefit mentioned above, no significant cross-reactivity or interference between Human TGF-β1 and analogues was observed after using the EliKine™ Human TGF-β1 ELISA Kit.
Some of the components of the kit include Human TGF-β1 microplate, Human TGF-β1 standard, Human TGF-β1 detect antibody, EliKine™ Streptavidin-HRP, and Standard diluent. The kit also consists of Assay buffer, HRP substrate, Stop solution, Wash buffer, and Plate covers.
About Abbkine Scientific Co., Ltd.
Abbkine Scientific Co., Ltd. was founded in 2012. Our mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. We're devoting to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, and drug discovery. Abbkine is delighted to offer reproducible and sensitive EliKine ELISA kits for quantification of cytokines, hormones and other proteins with sandwich or competitive ELISA methods, individually.
2017年12月29日星期五
Abbkine announces the release of EliKine™ Human TGF-β1 ELISA Kit
2017年12月13日星期三
LinKine™ FITC Labeling Kit becomes a new member to Conjugation kits family
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Abbkine Scientific has launched its brand-new product LinKine™ FITC Labeling Kit recently. This product is designed for preparing FITC conjugates directly from proteins, peptides, and other ligands that contain a free amino group. The high sensitivity and excellent specificity make this product become unique.
Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. FITC is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. This derivative is reactive towards nucleophiles including amine and sulfhydryl groups on proteins. FITC has excitation and emission spectrum peak wavelengths of approximately 492 nm/520 nm, giving it a green color.
The LinKine™ FITC Labeling Kit is also known as LinKine™ FITC Conjugation Kit which has many irreplaceable benefits. The kit includes purification columns, ensuring rapid and efficient removal of non-reacted dye and excellent protein recovery. Another featured benefit is the Single-use fluors. The featured FITC Conjugation Kit contains single-use vials of reagent. Since the product has been officially launched to LinKine™ Conjugation kits family, we can hope for the raising sales volume because of its high quality and competitive price.
The Kit consists of three parts- Activated FITC solution, FITC labeling solution, Purification column, which makes the kit easy to use. But you should pay attention to that kit components should be stored at 4˚C for 6 months and the initial concentration of the molecules to be labelled should not less than 2mg/ml. The issue of LinKine™ FITC Labeling Kit is a revolutionary step for Abbkine.
About Abbkine Scientific Co., Ltd.
Abbkine Scientific Co., Ltd. was founded in 2012. Our mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. We're devoting to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, and drug discovery. Abbkine is delighted to offer reproducible and sensitive EliKine ELISA kits for quantification of cytokines, hormones and other proteins with sandwich or competitive ELISA methods, individually.
2017年12月8日星期五
Abbkine Scientific announces the launch of LinKine™ HRP Labeling Kit
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Abbkine injected a large amount of human and financial resources in this year to develop a series of LinKine™ kits coupling labeling kits, which largely strengthen the core competitiveness of Abbkine products. The features and benefits of this series of kits are obvious. Firstly, it provides an optimized project for experiement. If you obey the standard experiment procedure, depending on the excellent pre-activated dye, Protein ratio and Patent solution formula, you can get the best activity or fluorescence. Secondly, this product is convenient to operate. You can achieve an ideal result normally by three steps, attaching the coupling group to the primary amine site of the antibody or other protein. Thirdly, we can supply customization service especially. By changing the molar ratio, reaction buffer, PH and other parameters, you can arrive at the different levels of coupling and activity. Abbkine holds a strong belief on the innovation and sustainability in this series of products.
As is known to all that horseradish peroxidase also referred to HRP is one of the most important enzymes obtained from a plant source. HRP is an important heme-containing enzyme that has been studied for more than a century. In recent years new information has become available on the three-dimensional structure of the enzyme and its catalytic intermediates, mechanisms of catalysis and the function of specific amino acid residues. It continues to attract the attention of researchers from a variety of disciplines because of its practical and commercial applications.
The featured conjugation Kit provides a simple and quick process to conjugate antibodies, peptides, proteinsand other molecules with free amine groups with HRP. This kit is absolutely a powerful supplement to Abbkine kit group. Here we’d like also to mention that horseradish peroxidase is a 44kDa glycoprotein with 6 lysine residues. The enzyme label can be visualized by chromogenic reactions.
The LinKine™ HRP Labeling Kit is mainly composed of three parts-Activated HRP conjugates solution, HRP labeling solution and HRP quencher powder. The featured benefits include High activity HRP, Convenient quantities and so on, which are extremely helpful to scientific research. This product is both high in quality and competitive in price and it deserves buying.
About Abbkine Scientific Co., Ltd.
Abbkine Scientific Co., Ltd. was founded in 2012. Our mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. We're devoting to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, and drug discovery. Abbkine is delighted to offer reproducible and sensitive EliKine ELISA kits for quantification of cytokines, hormones and other proteins with sandwich or competitive ELISA methods, individually.
2017年11月22日星期三
EliKine™ Human TNF-α ELISA Kit – the latest revolutionary scientific research kit
Human TNF-α ELISA Kit is the latest product from Abbkine Scientific Research Company. As part of the company’s plans to ease the research process and ensure that better results are gotten in record time, the company made the announcement to official launch the Human TNF alpha ELISA Kit on the market.The ELISA Kit is particularly designed to allow for the easy detection of Human TNF-α. Consequently, investigators, researchers and other such users of the product can easily get their desired results in record time. This also ensures that better results are gotten.
EliKine™ Human TNF-α ELISA Kit employs a two-site sandwich ELISA to quantitate TNF-α in samples, it has high sensitivity and excellent specificity for detection of Human TNF-α. No significant cross-reactivity or interference between Human TNF-α and analogues was observed.
The recent launch of the Human TNF-α ELISA Kit has been described by many as a revolutionary introduction to the industry. With a high sensitivity and excellent specificity for detection of Human TNF-α, the product stands above its peers on the market.
EliKine™ Human TNF alpha ELISA Kit includes Human TNF-α microplate, Human TNF-α standard, Human TNF-α detect antibody, EliKine™ Streptavidin-HRP, Standard diluen, Assay buffer, HRP substrate, Stop solution, Wash buffer, Plate covers. The kit has a calibration range of 7.8 pg/ml-500 pg/ml and uses the Colorimetric detection method, with a detection limit of 4 pg/mL.
About Abbkine ELISA Kit
Abbkine Inc. is a company founded by a team of scientists and marketing experts in the field of life science. Founded in 2012 and headquartered in China. Our mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health, we newly launched EliKine™ series of ELISA kits exert high sensibility and specificity, with a comprehensive selection of ELISA kits available for the quantification of cytokines, hormones and other proteins, to meet your multiple experiment demands.
2017年11月17日星期五
Abbkine Scientific announces new release of the EliKine™ Human FSH ELISA Kit
Abbkine Scientific Co., Ltd. has announced the official release of its new science research kit, the EliKine™ Human FSH ELISA Kit, designed to ease the research process and ensure investigators are more effective with their operations.Follicle-stimulating hormone (FSH) is a gonadotropin, a glycoprotein polypeptide hormone, it is also called FSH-alpha, FSHA, etc. FSH plays an important role in regulating the development, growth, pubertal maturation and reproductive processes of human being. FSH is often used in the treatment of infertility, especially during IVF. In some cases, it is also used for anovulation and reversal.
The EliKine™ Human FSH ELISA Kit is newly released by Abbkine Scientific which is a leading biotechnology company based in China, it includes Human FSH microplate, Human FSH standard, Human FSH detect antibody, HRP substrate A, HRP substrate B, Stop solution, Wash buffer,Plate covers. The Human FSH Kit has high sensitivity and excellent specificity for detecting human FSH and also has showed no significant cross-reaction or interference between human FSH and analogues.
Other features and benefits of Human FSH ELISA Kit include colorimetric detection method, multiple steps standard sandwich ELISA assay with a working time of 2 to 3 hours and a calibration range of 2 IU/L-70 IU/L.
The FSH kit is only for research use and is not intended for use in human or clinical diagnosis.
About Abbkine Scientific Co., Ltd.
Abbkine Scientific Co., Ltd. was founded in 2012. Our mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. We're devoting to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, and drug discovery. Abbkine is delighted to offer reproducible and sensitive EliKine ELISA kits for quantification of cytokines, hormones and other proteins with sandwich or competitive ELISA methods, individually.
2017年11月15日星期三
Rabbit Polyclonal Antibody Production Protocol (70-Day)
Standard 70-day rabbit immunization protocol for rabbit polyclonal antibody production
Abbkine has offered polyclonal antibody manufacturing services for more than four decades. Our expertise includes using a variety of different hosts for the development of polyclonal antibodies, including the most commonly used New Zealand white rabbits. Goats, sheep, rats, mouse and donkeys are also often selected.
This 2-Rabbit 70-Day protocol is optimized to produce antiserum in the shortest possible time. It is the most affordable option, popular among individual academic researchers.
The default immunization schedule uses two NZW SPF rabbits (New Zealand White rabbits that are Specific Pathogen-Free); additional rabbits are easily added to the standard order for a modest cost. Injections are subcutaneous (SQ) as emulsions in Complete Freund's Adjuvant (CFA) or Incomplete Freund's Adjuvant (IFA); alternative adjuvants can be used if requested. Continuation of rabbits after Day 60 is offered on a month-to-month basis. A brief protocol is listed below.
Antigen preparation, such as peptide synthesis and/or immunogen conjugation, occurs before Day 0. Injection amounts are given for a conjugated peptide antigen (e.g., KLH) or protein immunogen; MAP-peptide antigens are injected at 0.5mg throughout. Protocol Days are approximate (± 2 days).
| Procedure | Protocol day | Description |
|---|---|---|
| Control serum collection | Day 0 | Pre-immune bleed (5 mL per rabbit) |
| Primary injection | Day 1 | Immunize with 0.50 mg of antigen in CFA, 10 SQ sites |
| 1st booster | Day 14 | Boost with 0.25 mg of antigen in IFA, 4 SQ sites |
| 2nd booster | Day 28 | Boost with 0.25 mg of antigen in IFA, 4 SQ sites |
| Serum collection | Day 35 | Bleed (~25 mL per rabbit) |
| 3rd Booster | Day 42 | Boost with 0.25 mg of antigen in IFA, 4 SQ sites |
| Serum collection | Day 56, 58 | Two bleeds (~50 mL total per rabbit) |
| ELISA and shipping | Day 60 | ELISA titration (results available online); Verify disposition of rabbits; decide to continue or terminate |
| Instruction due date | Day 72 | If no animal instructions are received, per diem charges will apply |
Deliverables (Day 60): 12 vials crude antibody sera
| ||
2017年11月12日星期日
EliKine™ Human CCL2/MCP-1 ELISA Kit Officially Released by Abbkine
Abbkine Scientific has officially announced the release of its EliKine™ Human CCL2 ELISA Kit. The product also is known as the Human MCP1 ELISA Kit which is unique for its high sensitivity and excellent specificity.The chemokine (C-C motif) ligand 2 (CCL2) is also referred to as monocyte chemoattractant protein 1 (MCP1) and small inducible cytokine A2. Other alternative names include MCP-1, HC11, MCAF, HSMCR30, SMC-CF, GDCF-2, SCYA2, monocyte chemoattractant protein-1, monocyte secretory protein JE. CCL2 is a small cytokine that belongs to the CC chemokine family. CCL2 recruits monocytes, memory T cells, and dendritic cells to the sites of inflammation produced by either tissue injury or infection. Abbkine newly launched EliKine™ Human CCL2/MCP-1 ELISA Kit exerts high sensibility and specificity for the quantification of Human CCL2/MCP-1 in various samples to CCL2 level determination.
The Human MCP1 ELISA Kit comes with different features and benefits that stand it out from its contemporaries on the market. This ELISA kit employs a two-site sandwich ELISA to quantitate CCL2 in samples, using colorimetric detection method. With a Sandwich ELISA (quantitative) assay type and working duration of between 3 and 5 hours depending on the operator, the kit looks to be a game changer in the industry.
This featured Human CCL2/MCP-1 ELISA Kit components that include Human CCL2 microplate, Avidin-HRP, HRP substrate, Wash buffer, and Stop solution, allows for easy use and manipulation, further endearing it to several investigators and researchers across the world. Our complete, ready-to-use ELISA Kits reduce assay time and variability and are available in either 1 or 10 pre-coated plate options.
About Scientific Co., Ltd.
Abbkine Scientific Co., Ltd. was founded in 2012. Our mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health, We're devoting to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, and drug discovery. Abbkine is delighted to offer reproducible and sensitive EliKine ELISA kits for quantification of cytokines, hormones and other proteins with sandwich or competitive ELISA methods, individually.