TraKine™ Pro Live-cell Fluorescence Tool-Perfect compatibility with long-term super-resolution fluorescence imaging

Combining genetic marker method with small and bright probes that can be controlled by light-controlled switches will be a very ideal method to develop molecular-level ultra-high resolution imaging in the future.

- Xiaowei Zhuang (Discoveries of novel cellular structures using STORM)

Inspired by academician Xiaowei Zhuang, Abbkine TraKine™ Pro series of fluorescent dyes came into being.
TraKine™ Pro is series of long-term super-resolution cell staining imaging portfolio for labeling subcellular structures of live and fixed cells. TraKine™ Pro proprietary excellent fluorescent dyes span the full UV-visible and near IR spectrum.

This release of the TraKine™ Pro series featured hot research targets, including,

Tubulin: One of the important components of eukaryotic cytoskeleton, plays an important role in maintaining cell morphology, cell division, signal transduction and material transport.
Lysosome & Mitochondrion: An important dynamic organelle, widely involved in many important cellular processes. Their dysfunction is related to many diseases, such as neurodegenerative diseases, cancer and cardiovascular diseases.
Nuclei: Controls the genetic characteristics of organisms. Cell metabolism is mainly carried out by controlling the synthesis of specific enzymes. Responsible for protein synthesis, cell division, growth and differentiation, and regulating gene integrity and gene expression.

* We accept product customization based on TraKine™ Pro technology.

 Preemptive attention to product parameters:

Product name	Cat#	Target	Ex/Em（nm）	Live or fixed cells	Microscope compatibility
TraKine™ Pro Live-cell Tubulin Staining kit (Green Fluorescence with Super Resolution)	KTC4100	Tubulin	500/520	Live cells	Fluorescence microscope，Confocal, STED, SIM, PALM, STORM, TIRF
TraKine™ Pro Live-cell Tubulin Staining kit (Deep Red Fluorescence with Super Resolution)	KTC4110	Tubulin	650/665	Live cells	Fluorescence microscope，Confocal, SIM, PALM, STORM, TIRF
TraKine™ Pro Live-cell Lysosome Staining kit (Green Fluorescence with Super Resolution)	KTC4200	Lysosome	494/517	Live& fixed cells	Fluorescence microscope，Confocal, STED, SIM, PALM, STORM, TIRF
TraKine™ Pro Live-cell Lysosome Staining kit (Deep Red Fluorescence with Super Resolution)	KTC4210	Lysosome	650/665	Live& fixed cells	Fluorescence microscope，Confocal, SIM, PALM, STORM, TIRF
TraKine™ Pro Live-cell Mitochondrion Staining kit (Deep Red Fluorescence with Super Resolution)	KTC4300	Mitochondrion	647/661	Live& fixed cells	Fluorescence microscope，Confocal, STED, SIM, PALM, STORM, TIRF
TraKine™ Pro Live-cell Nuclei Staining kit (Green Fluorescence with Super Resolution)	KTC4500	Nuclei	494/517	Live& fixed cells	Fluorescence microscope，Confocal, STED, SIM, PALM, STORM, TIRF
TraKine™ Pro Live-cell Nuclei Staining kit (Deep Red Fluorescence with Super Resolution)	KTC4510	Nuclei	650/665	Live& fixed cells	Fluorescence microscope，Confocal, SIM, PALM, STORM, TIRF

Confocal: confocal microscopes

STED: stimulated emission depletion

SIM: structured-illumination microscopy

PALM: photoactivation localization microscopy

STORM: stochastic optical reconstruction microscopy

TIRF,: total internal reflection fluorescence imaging

If you only limit the observation of cells on the plane instead of the three-dimensional structure of cells, you will always see only consistent but featureless clouds of color. Using unique TraKine™ Pro series dyes, combined with super-resolution microscopy technology, the real cell structure and fresh and living colors spring up!

The choice of beauty or mediocrity depends entirely on the horizon. Feel the unique charm of TraKine™ Pro series dyes together.

 

SIM imaging results of U2OS cells using Abbkine TraKine™ Pro Live-cell Tubulin Staining kit (Green Fluorescence with Super Resolution). It can be seen that the kit can specifically label microtubule structures in living cells and perform ultra-high resolution imaging.

 

SIM imaging results of U2OS cells using Abbkine TraKine™ Pro Live-cell Lysosome Staining kit (Deep Red Fluorescence with Super Resolution). It can be seen that the kit can specifically label Lysosome structures in living cells and perform ultra-high resolution imaging.

 

SIM imaging results of U2OS cells using Abbkine TraKine™ Pro Live-cell Mitochondrion Staining kit (Deep Red Fluorescence with Super Resolution).

 

About Abbkine Scientific Co., Ltd.

 Abbkine serves global scientists in the field of proteomics and cytology and is committed to the innovation and development of various scientific reagents related to proteomics and cytology, expecting to accelerate the pace of life science research and drug discovery. Proteomics products cover the preparation of samples (protein extraction, purification, coupling), protein quantification, antibodies and kits for protein detection. Cytology products involve cytokines (cell culture), cell status detection, cell staining, organelle extraction, cell metabolism and cytopathology reagents (kits). Abbkine relies on the product portfolio and unique marketing support as the main market strategy and product innovation mode, with ultimate aim to facilitate your research career.

Just choose Abbkine products for your interleukin study

 

What’s interleukin? What important roles do they play ?

 

Interleukins (ILs) are a group of cytokines (secreted proteins and signal molecules) that were first seen to be expressed by white blood cells (leukocytes), The name "interleukin" was chosen in 1979, during the Second International Lymphokine Workshop in Switzerland. An Arabic number is added after the name to distinguish them, such as IL-1, IL-2..., the newly determined factors are named in turn.

Interleukin is a kind of cytokines secreted by macrophages, lymphocytes and other cells. Its main biological functions involve the participation in immune response and immune regulation, constitution of the molecular systems that transmit signals between cells, and so on. Different interleukins have different sources and functions, and some can be produced by different kinds of immune cells. An interleukin can also actin on a variety of cells.

At least 38 interleukins have been found currently, which are named IL-1 ~IL38. They play important roles in the maturation, activation, proliferation and immune regulation of immune cells. In addition, they also participate in a variety of physiological and pathological responses of the body. Deficiency of some rare interleukins can result in autoimmune diseases or immunodeficiency.

 



 

Latest research letters of interleukin may give you new thoughts

 

1. Scientists from University of Sydney studied the role of IL-17 in its role in diabetic nephropathy. They concluded that absence of IL-17 signalling is protective against streptozotocin-induced diabetic nephropathy, thus implying a pro-inflammatory role of IL-17 in its pathogenesis. Targeting the IL-17 axis may represent a novel therapeutic approach in the treatment of this disorder.


2. University of Chinese Academy of Sciences first detected that FBXO38 mediates PD-1 ubiquitination and regulates anti-tumour immunity of T cell. IL-2 therapy rescued Fbxo38 transcription and therefore downregulated PD-1 levels in PD-1+ T cells in mice. Data indicated that FBXO38 regulates PD-1 expression and highlight an alternative method to block the PD-1 pathway.


3. Researchers from Albany Medical College elucidated a signaling cascade underlying intestinal fibrosis in which altered mTOR/autophagy in CX3Cr1+ mononuclear phagocytes up-regulates the IL-23/IL-22 axis, leading to an excessive fibrotic response. Thus, our findings suggest that this cascade could be a therapeutic target for alleviation of CD fibrosis.


4. Study suggested IL-17RB as a potential early intervening target in H. pylori infection. This study defines a negative regulatory network involving IL-17E, GECs, IL-17RB, CD11b+CD11c− myeloid cells, and Reg3a in the early-phase of H. pylori infection, which results in an impaired host defense within the gastric microenvironment.


5. Through the study of short-chain fatty acid pentanoate suppresses autoimmunity by modulating the metabolic-epigenetic crosstalk in lymphocytes, researchers found that by enhancing IL-10 production and suppressing Th17 cells, the SCFA pentanoate might be of therapeutic relevance for inflammatory and autoimmune diseases.


6. Study showed that Human IFN-γ immunity to mycobacteria is governed by both IL-12 and IL-23. Human IL-12 and IL-23 are both required for optimal IFN-γ–dependent immunity to mycobacteria, both individually and much more so cooperatively.

 

What related products can Abbkine offer ?

 

1. Recombinant proteins

Abbkine provides a wide range of recombinant protein products including interleukin, chemokines, interferons, transforming growth factor (TNF), tumor necrosis factor and other cytokines, for the study of cancer, apoptosis, endocrinology, immunology, neuroscience, protease and stem cell research.

The proteins are rigorously tested to meet the research and development demand for excellent quality, uncompromising biological activity at competitive prices, with low endotoxin, and lyophilized powder is available. Part of items are as below.

Product name	Cat#	Expression	Purity
Human IL-4 protein	PRP100273	E. coli	> 92 %
Human IL-1 alpha protein	PRP100048	Human Cells	> 95 %
Mouse IL-17 protein	PRP100491	E. coli	> 97 %
Human IL-6 protein	PRP100120	E. coli	> 95 %
Human IFN-beta protein	PRP100183	CHO Stable Cells	> 95 %

 

2. Crown primary antibodies

Abbkine primary antibodies arget to multiple research areas containing well known signaling pathway, neuroscience, cytoskeleton, cancer, epigenetics, etc. Comprehensive validation, various application, and reliable quality makes Abbkine you first choice. Some interleukin and interleukin receptor associated antibodies are as follows.

Product name	Cat#	Application
IL-1β Polyclonal Antibody	ABP52932	ELISA, IHC-p, WB
IL-1β Polyclonal Antibody	ABP51611	ELISA, IHC-p, WB
IL-6 Polyclonal Antibody	ABP53079	ELISA, IHC-p, WB
IL-8 Polyclonal Antibody	ABP52884	ELISA, IF, IHC-p, WB
IL-20Rα Polyclonal Antibody	ABP51613	ELISA, IHC-p, WB

 

Result example: Immunohistochemical analysis of paraffin-embedded rat lung tissue.IL-1β Polyclonal Antibody was diluted at 1:200 (4°C, overnight).

3. ELISA kits

Abbkine offers a rich portfolio of ELISA kits covering most of research targets (cancer, diabetes, obesity, hormones, food, cytokines, etc.) and multiple species (Human, Mouse, Rat, Dog, Pig, Chicken, Rabbit, Bovine, Canine, Sheep, Fish). Abbkine EliKine™ series of ELISA kits exert high sensibility and specificity, with a comprehensive selection of ELISA kits available for the quantification of cytokines, hormones and other proteins, to meet your multiple experiment demands, with high cost performance and popular with customers. Some indicators are recommended as follows.

Product name	Cat#	Detection Range
EliKine™ Human IL-1β ELISA Kit	KET6013	7.8 pg/ml-500 pg/ml
EliKine™ Human IL-6 ELISA Kit	KET6017	3.125 pg/ml-200 pg/ml
EliKine™ Human IL-18 ELISA Kit	KET6023	156 pg/mL- 10000 pg/mL
EliKine™ Mouse IL-6 ELISA Kit	KET7009	7.8 pg/mL-500 pg/mL
EliKine™ Rat IL-6 ELISA Kit	KET9004	250pg/mL-16000 pg/mL

 

About Abbkine Scientific Co., Ltd.

Abbkine serves global scientists in the field of proteomics and cytology and is committed to the innovation and development of various scientific reagents related to proteomics and cytology, expecting to accelerate the pace of life science research and drug discovery. Proteomics products cover the preparation of samples (protein extraction, purification, coupling), protein quantification, antibodies and kits for protein detection. Cytology products involve cytokines (cell culture), cell status detection, cell staining, organelle extraction, cell metabolism and cytopathology reagents (kits). Abbkine relies on the product portfolio and unique marketing support as the main market strategy and product innovation mode, with ultimate aim to facilitate your research career!

Abbkine invites you to visit AACR 2019, Atlanta, Georgia!

Abbkine will exhibit at American Association for Cancer Research (AACR) 2019, Atlanta, Georgia

Abbkine invites you to visit AACR 2019, Atlanta, Georgia!

Abbkine latest products and services will be displayed at the exhibition. There always be one type can flash into your eyes.

 

American Association for Cancer Research (AACR) 2019

Date: March 31 to April 3, 2019

Booth: #4454

Address: Georgia World Congress Center in Atlanta, Georgia

 

About: The AACR Annual Meeting program covers the latest discoveries across the spectrum of cancer research—from population science and prevention; to cancer biology, translational, and clinical studies; to survivorship and advocacy—and highlights the work of the best minds in research and medicine from institutions all over the world.

 

Welcome to visit our booth #4454.

 

Abbkine will exhibit at American Association for Cancer Research (AACR) 2019, Atlanta, Georgia

Abbkine invites you to visit AACR 2019, Atlanta, Georgia!

Abbkine latest products and services will be displayed at the exhibition. There always be one type can flash into your eyes.

American Association for Cancer Research (AACR) 2019

Date: March 31 to April 3, 2019

Booth: #4454

Address: Georgia World Congress Center in Atlanta, Georgia

About: The AACR Annual Meeting program covers the latest discoveries across the spectrum of cancer research—from population science and prevention; to cancer biology, translational, and clinical studies; to survivorship and advocacy—and highlights the work of the best minds in research and medicine from institutions all over the world.

Welcome to visit our booth #4454.

Happy Chinese Lunar New Year of Pig

Dear customers,

We would like to take this opportunity to thank you for your kind support all the time. Please kindly be advised that our company will be closed from January 30, 2019 to February 11, 2019, in observance of the Chinese traditional festival, Spring Festival.

Hence our office and warehouse are closed during the above period. Please feel free to contact us by sending E-mail to service@abbkine.com. Orders will be accepted but not be processed until February 12, 2019, the first business day after the Spring Festival. Sorry for any inconvenience caused.

Happy New Year to you and your family!

Abbkine Scientific Co., Ltd.

About Abbkine Scientific Co., Ltd.

Abbkine serves global scientists in the field of Proteomics and Cytology, and is committed to the innovation and development of various proteomics and cytology related research reagents, with a view to becoming a key promoter in the fields of life science research and drug research and development, etc. Proteomics products cover from sample preparation (protein extraction, purification and coupling), protein quantification to antibodies and kits for protein detection; Cytological products include cytokines for cell culture, cell state detection, staining, organelle extraction, cell metabolism and cell pathology. Abbkine relies on its product portfolio and unique marketing support as its main marketing strategy and product innovation model, only to help your research career.

Featured Cell Cycle Staining Kit from Abbkine scientific

Cell cycle analysis by quantitation of DNA content was one of the earliest applications of flow cytometry. The DNA of mammalian, yeast, plant or bacterial cells can be stained by a variety of DNA binding dyes. As cells progress through the cell cycle, cells in the G1 phase have one set of paired chromosomes and are uniform with respect to DNA content. On the other hand, the amount of DNA begins to double during S phase, so that the amount of DNA is between one and two times the amount in G1. Cells in G2/M phase have double the amount of DNA compared to cells in G1 and two sets of paired chromosomes.



Abbkine Cell Cycle Staining Kit utilizes a nuclear dye, the binding of which to nucleic acids in the cell results in fluorescence signal, which is proportional to cellular DNA content, providing a convenient and accurate determination of the percentage of cells in each phase of the cell cycle. The appropriate observe channel is Ex/Em=535/615 nm.

Tips

1. It is very important that the cells are fixed into a single cell suspension. Cells can tend to clump during fixation. Very slow, drop-wise addition of the initial volume of 70% ethanol while gently vortexing helps prevent cells from clumping.

2. 70% ethanol should not be made with PBS as this causes protein precipitation on fixation. Use 70 parts absolute ethanol to 30 parts distilled water.

Product Name	Cat No.	Details
Cell Cycle Staining Kit	KTA2020	Click here

 

Abbkine serves global scientists in the field of Proteomics and Cytology, and is committed to the innovation and development of various proteomics and cytology related research reagents, with a view to becoming a key promoter in the fields of life science research and drug research and development, etc. Proteomics products cover from sample preparation (protein extraction, purification and coupling), protein quantification to antibodies and kits for protein detection; Cytological products include cytokines for cell culture, cell state detection, staining, organelle extraction, cell metabolism and cell pathology. Abbkine relies on its product portfolio and unique marketing support as its main marketing strategy and product innovation model, only to help your research career.

About Abbkine Scientific Co., Ltd.

Abbkine originated from the United States, made in China and served the world. Combining the great advantages of American technology and Chinese manufacturing, Abbkine is committed to providing innovative, high-quality and affordable experimental kits, proteins, antibodies and other research tools for scientists in the field of Proteomics and Cytology worldwide, with a view to becoming a key promoter in the fields of life science research development and drug research and development.

Abbkine newly launched TUNEL Apoptosis Detection Kit (Orange Fluorescence)

Apoptosis is a form of programmed cell death that occurs in multicellular organisms. Biochemical events lead to characteristic cell changes (morphology) and death. These changes include blebbing, cell shrinkage, nuclear fragmentation, chromatin condensation, chromosomal DNA fragmentation, and global mRNA decay. A failure of cells to undergo apoptosis is a common feature of many cancers thus investigation of apoptosis inducing mechanisms is of particular importance for cancer research and requires suitable detection methods.

A hallmark of late apoptosis is extensive genomic DNA fragmentation that generates a multitude of DNA double-strand breaks with accessible 3'-hydroxyl (3'-OH) groups. Terminal deoxynucleotidyl transferase dUTP Nick End Labeling (TUNEL) assay is a method for detecting DNA fragmentation by labeling the 3’-OH termini in the double-strand DNA breaks generated during apoptosis. The TUNEL assay relies on the presence of nicks in the DNA which can be identified by TdT, an enzyme that catalyzes the addition of dUTPs that are labeled with fluorescein.

Abbkine TUNEL Apoptosis Detection Kit (Orange Fluorescence) provides all the essential components with an optimized assay protocol, suitable for fluorescence microplate reader, fluorescence microscope, or flow cytometer. Its signal can be easily detected (Ex/Em = 555 nm/565 nm).



 

Below are details of Abbkine TUNEL Apoptosis Detection Kits :

Product Name	Cat No.	Ex/Em	Details
TUNEL Apoptosis Detection Kit (Green Fluorescence)	KTA2010	490/520nm	Click here
TUNEL Apoptosis Detection Kit (Orange Fluorescence)	KTA2011	555/565 nm	Click here

 

Abbkine serves global scientists in the field of Proteomics and Cytology, and is committed to the innovation and development of various proteomics and cytology related research reagents, with a view to becoming a key promoter in the fields of life science research and drug research and development, etc. Proteomics products cover from sample preparation (protein extraction, purification and coupling), protein quantification to antibodies and kits for protein detection; Cytological products include cytokines for cell culture, cell state detection, staining, organelle extraction, cell metabolism and cell pathology. Abbkine relies on its product portfolio and unique marketing support as its main marketing strategy and product innovation model, only to help your research career.

About Abbkine Scientific Co., Ltd.

Abbkine originated from the United States, made in China and served the world. Combining the great advantages of American technology and Chinese manufacturing, Abbkine is committed to providing innovative, high-quality and affordable experimental kits, proteins, antibodies and other research tools for scientists in the field of Proteomics and Cytology worldwide, with a view to becoming a key promoter in the fields of life science research development and drug research and development.