Abbkine Scientific announces new product launch - IFKine™ Green Donkey Anti-Mouse IgG

The IFKine™ Green Donkey Anti-Mouse IgG is the latest member of the Abbkine family, with the company recently announcing the official launch of the product. The Green dye Donkey Anti-Mouse IgG is one the antibodies from the scientific research giant designed to make researches easier, more effective and result-oriented.

Otherwise known as Dylight 488 Antibody, the product is designed to absorb light maximally at 493 nm and fluoresce with a peak around 518 nm. Like other antibodies from Abbkine, the IFKine™ Green Antibody as it is also known is hosted by an animal and in this case, the donkey.

The antibody is recommended for all immunofluorescence procedures requiring a green-fluorescing dye, with the immunogen being Mouse IgG whole molecule. Available in liquid solution, the product can be applied in FCM, ICC, IF.

The antibody is polyclonal with mouse reactivity and has been affinity purified using solid phase Mouse IgG (H&L) with finally > 95% purity based on SDS-PAGE. One of the major benefits of this series of antibody is that they make sure the best fluorescent performance, with its donkey host and other species of serum/IgG absorbed making IFKine™ secondary antibodies ideal for use in fluorence staining, especially in fluorence multiple labeling.

The antibody reacts with whole molecule mouse IgG and reacts with light chains of all other mouse immunoglobulins.

About Abbkine Scientific Co. Ltd

Abbkine Scientific Co. Ltd headquartered in China. Founded in 2012, the company was set up by a team of scientists and marketing experts in the field of life science. The company has been able to combine cutting edge technology from United States with China's manufacturing engineering and cost advantages, providing innovative, high quality assay kits, recombinant proteins, antibodies to enhance life science.

EliKine™ Human TNF-α ELISA Kit review

TNFa is synthesized as a 26 kDa, type II transmembrane protein that is 233 amino acids in length. It contains a 30 amino acid (aa) cytoplasmic domain, a 26 aa transmembrane segment, and a 177 aa extracellular region. TNFa is assembled intracellularly to form a transmembrane, non-covalently-linked homotrimeric protein. The 157 aa residue soluble form of TNFa (sTNF-αis released from the C-terminus of the transmembrane protein through the activity of TNFa-converting enzyme (TACE), a membrane -bound disintegrin metalloproteinase.

EliKine™ Human TNF-α ELISA Kit employs a two-site sandwich ELISA to quantitate TNF-α in samples. An antibody specific for TNF-α has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNF-α present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNF-α is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNF-α bound in the initial step. The color development is stopped and the intensity of the color is measured.

EliKine™ Human TNF-α ELISA Kit was purchased to detect TNFA in Human serum. It meets our requirements very well. The detection range of this kit is from 7.8 pg/ml to 500 pg/ml. The minimum detectable dose of Human TNFA is typically less than 4 pg/mL. No significant cross-reactivity or interference was found during the test. The design of 12 strips of 8 wells 96 well polystyrene microplates is very flexible. The unused wells can be stored at 2-8 ˚C for up to one month. Compared to other brand, the price is reasonable.

Minimum information about a single amplified genome (MISAG) and a metagenome-assembled genome (MIMAG) of bacteria and archaea

Topics overview: REAP-seq, miRNA expression and promoters in primary mammalian cells, Control of stereochemistry substantially increases the efficacy of antisense oligonucleotides, atlas of B-cell clonal lineages, MISAG and MIMAG of bacteria and archaea.

1. Multiplexed quantification of proteins and transcripts in single cells

Vanessa M Peterson at Merck & Co., Inc. in Massachusetts, USA and his colleagues present a tool to measure gene and protein expression levels in single cells with DNA-labeled antibodies and droplet microfluidics. Using the RNA expression and protein sequencing assay (REAP-seq), they quantified proteins with 82 barcoded antibodies and >20,000 genes in a single workflow. They used REAP-seq to assess the costimulatory effects of a CD27 agonist on human CD8+ lymphocytes and to identify and characterize an unknown cell type.

Read more, please click http://www.nature.com/nbt/journal/vaop/ncurrent/full/nbt.3973.html

2. An integrated expression atlas of miRNAs and their promoters in human and mouse

MicroRNAs (miRNAs) are short non-coding RNAs with key roles in cellular regulation. As part of the fifth edition of the Functional Annotation of Mammalian Genome (FANTOM5) project, Derek de Rie at RIKEN Center for Life Science Technologies in Yokohama, Japan and his colleagues created an integrated expression atlas of miRNAs and their promoters by deep-sequencing 492 short RNA (sRNA) libraries, with matching Cap Analysis Gene Expression (CAGE) data, from 396 human and 47 mouse RNA samples. Promoters were identified for 1,357 human and 804 mouse miRNAs and showed strong sequence conservation between species. They also found that primary and mature miRNA expression levels were correlated, allowing us to use the primary miRNA measurements as a proxy for mature miRNA levels in a total of 1,829 human and 1,029 mouse CAGE libraries. They thus provide a broad atlas of miRNA expression and promoters in primary mammalian cells, establishing a foundation for detailed analysis of miRNA expression patterns and transcriptional control regions.

Read more, please click http://www.nature.com/nbt/journal/vaop/ncurrent/full/nbt.3947.html

3. Control of phosphorothioate stereochemistry substantially increases the efficacy of antisense oligonucleotides

Whereas stereochemical purity in drugs has become the standard for small molecules, stereoisomeric mixtures containing as many as a half million components persist in antisense oligonucleotide (ASO) therapeutics because it has been feasible neither to separate the individual stereoisomers, nor to synthesize stereochemically pure ASOs. Here Naoki Iwamoto at Wave Life Sciences in Massachusetts, USA and his colleagues report the development of a scalable synthetic process that yields therapeutic ASOs having high stereochemical and chemical purity. Using this method, they synthesized rationally designed stereopure components of mipomersen, a drug comprising 524,288 stereoisomers. They demonstrate that phosphorothioate (PS) stereochemistry substantially affects the pharmacologic properties of ASOs. They report that Sp-configured PS linkages are stabilized relative to Rp, providing stereochemical protection from pharmacologic inactivation of the drug. Further, they elucidated a triplet stereochemical code in the stereopure ASOs, 3′-SpSpRp, that promotes target RNA cleavage by RNase H1 in vitro and provides a more durable response in mice than stereorandom ASOs.

Read more, please click http://www.nature.com/nbt/journal/vaop/ncurrent/full/nbt.3948.html

4. An atlas of B-cell clonal distribution in the human body

B-cell responses result in clonal expansion, and can occur in a variety of tissues. To define how B-cell clones are distributed in the body, Wenzhao Meng at Perelman School of Medicine, University of Pennsylvania in Pennsylvania, USA and his colleagues sequenced 933,427 B-cell clonal lineages and mapped them to eight different anatomic compartments in six human organ donors. They show that large B-cell clones partition into two broad networks—one spans the blood, bone marrow, spleen and lung, while the other is restricted to tissues within the gastrointestinal (GI) tract (jejunum, ileum and colon). Notably, GI tract clones display extensive sharing of sequence variants among different portions of the tract and have higher frequencies of somatic hypermutation, suggesting extensive and serial rounds of clonal expansion and selection. Their findings provide an anatomic atlas of B-cell clonal lineages, their properties and tissue connections. This resource serves as a foundation for studies of tissue-based immunity, including vaccine responses, infections, autoimmunity and cancer.

Read more, please click http://www.nature.com/nbt/journal/vaop/ncurrent/full/nbt.3942.html

5. Minimum information about a single amplified genome (MISAG) and a metagenome-assembled genome (MIMAG) of bacteria and archaea

Robert M Bowers at Department of Energy Joint Genome Institute in California, USA and his colleagues present two standards developed by the Genomic Standards Consortium (GSC) for reporting bacterial and archaeal genome sequences. Both are extensions of the Minimum Information about Any (x) Sequence (MIxS). The standards are the Minimum Information about a Single Amplified Genome (MISAG) and the Minimum Information about a Metagenome-Assembled Genome (MIMAG), including, but not limited to, assembly quality, and estimates of genome completeness and contamination. These standards can be used in combination with other GSC checklists, including the Minimum Information about a Genome Sequence (MIGS), Minimum Information about a Metagenomic Sequence (MIMS), and Minimum Information about a Marker Gene Sequence (MIMARKS). Community-wide adoption of MISAG and MIMAG will facilitate more robust comparative genomic analyses of bacterial and archaeal diversity.

Read more, please click http://www.nature.com/nbt/journal/v35/n8/full/nbt.3893.html

Peroxiredoxin 1 Monoclonal Antibody – the latest addition to the Abbkine family

Abbkine Scientific Company Limited, a Chinese-based research company has announced the addition of a new member of its antibody family, the Peroxiredoxin 1 Monoclonal Antibody. Also known as the PRDX1 antibody, the Peroxiredoxin-1 antibody is designed to make the process of investigation and research easier for scientists, helping to yield more results.

The antibody is formulated using the PRDX1, a substance that encodes a part of the peroxiredoxin family of antioxidant enzymes that reduce alkyl hydroperoxides and hydrogen peroxide. Peroxiredoxin 1 has also been identified to function as an antioxidant protection in cells, contributing to the antiviral activity of CD8(+) T-cells.

The product is a Proliferation-associated gene protein antibody, helping to fight possible development or progression of cancer. Available in liquid solution, the antibody has human, mouse and rat reactivity, with applications including IF, IHC-p, and WB.

The optimal working dilutions of the monoclonal antibody can be determined experimentally by the research or investigator as the case may be. However, the suggested starting dilutions are WB: 1:1000-3000, IF: 1:100-200.

The antibody was made using state-of-the-art technology as it has been affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

The product can remain stable for one year if stored under the right condition at -20°C. Abbkine has declared that the product is solely for research purpose and should not be used in human or for diagnostic purposes.

About Abbkine Scientific Co. Ltd

Abbkine Scientific is a scienitific research company originally established in California, USA in 2012 before moving its headquarters to China due to increasing demand for its products from Asia Pacific. The company through the combination of China’s manufacturing engineering and cost advantages and cutting edge technology from United States has been able to provide state-of-the-art scientific research solutions that include high quality assay kits, recombinant proteins, and antibodies.

EliKine™ Human CD54 ELISA Kit review

ICAM-1 (intercellular adhesion molecule-1), also known as CD54, is a transmembrane protein that is upregulated on endothelial and epithelial cells at sites of inflammation. It mediates the vascular adhesion and paracellular migration of leukocytes expressing activated LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). It also binds several non-integrin ligands including CD43/Sialophorin, Fibrinogen, Hyaluronan, rhinoviruses, and Plasmodium falciparum-infected erythrocytes. Soluble ICAM-1 promotes angiogenesis and serves an indicator of vascular endothelial cell activation or damage. Elevated levels of soluble ICAM-1 are associated with cardiovascular disease, type 2 diabetes, organ transplant dysfunction, oxidant stress, increased abdominal fat mass, hypertension, liver disease, certain malignancies, and cerebral malaria.

Human CD54 ELISA Kit employs a two-site sandwich ELISA to quantitate CD54 in samples. An antibody specific for CD54 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CD54 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD54 is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD54 bound in the initial step. The color development is stopped and the intensity of the color is measured.

We always see many brands who provide Elisa Kits, so it maybe a little difficult to choose an appropriate one. Abbkine maybe a good selection for you. I ever purchased several antibodies form Abbkine, and have to say this is a trustworthy brand. Recently, I ordered a EliKine™ Human CD54 ELISA Kit and want to detect the CD54 in the serum sample. Experiments show that Abbkine ELISA kit has high sensitivity and excellent specificity. No significant cross-reactivity or interference between Human CD54 and analogues was observed. I really appreciate this experience, I will continue to support Abbkine products.

EliKine™ Rat TNF-α ELISA Kit Review

TNFa is synthesized as a 26 kDa, type II transmembrane protein that is 233 amino acids in length. It contains a 30 amino acid (aa) cytoplasmic domain, a 26 aa transmembrane segment, and a 177 aa extracellular region. TNFa is assembled intracellularly to form a transmembrane, non-covalently-linked homotrimeric protein. The 157 aa residue soluble form of TNFa (sTNF-αis released from the C-terminus of the transmembrane protein through the activity of TNFa-converting enzyme (TACE), a membrane -bound disintegrin metalloproteinase. Rat cells known to express TNF-αinclude B cells, colonic columnar epithelial cells, NK and CD3 CD56 hepatic natural T cells, macrophages, monocytes and monocyte-derived dendritic cells, CD4 and CD8 T cells, mast cells, neutrophils, keratinocytes, plasma cells, and adipocytes.

EliKine™ Rat TNF-α ELISA Kit employs a two-site sandwich ELISA to quantitate TNF-α in samples. An antibody specific for TNF-α has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNF-α present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNF-α is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNF-α bound in the initial step. The color development is stopped and the intensity of the color is measured.

EliKine™ Rat TNF-α ELISA Kit was purchased to detect TNFA in Rat serum. It meets our requirements very well. The detection range of this kit is from 31.25 pg/ml to 2000 pg/ml. The minimum detectable dose of Rat TNFA is typically less than 16 pg/mL. No significant cross-reactivity or interference was found during the test. The design of 12 strips of 8 wells 96 well polystyrene microplates is very flexible. The unused wells can be stored at 2-8 ˚C for up to one month.

NFkB p65 Monoclonal Antibody is the latest antibody from Abbkine Scientific

Leading scientific research company, Abbkine Scientific Co. Ltd has announced the launch of another antibody named NFkB p65 Monoclonal Antibody. The product joins the long list of scientific products manufactured by Abbkine to make scientific research easier and more effective.

Nuclear factor NF-kappa-B p65 subunit antibody is made for the easy detection of endogenous p65 proteins, one feature that has stand it out from the competition.

The company is famous for producing different scientific research solutions and the Nuclear Factor NF Kappa B p65 Subunit antibody otherwise known as relA antibody is not an exception. The antibody comes with different features that have made it endearing to most researchers and investigators.

The p65 NF kappaB antibody has a recombinant protein immunogen, with applications that include IF, IHC-p, IP, WB. Abbkine Scientific has subsequently advised investigators and researchers to determine the optimal working dilutions while suggesting starting dilutions of WB: 1:500-2000, IP:1:200.

The antibody is monoclonal and has been affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. Available in a liquid solution, Nuclear factor NF-kappa-B p65 subunit antibody has a concentration of 1 mg/ml.

If stored at a stable condition of -20°C, the product can remain for up to a year from the date of shipment. The product is exclusively made for research use only and should not be used in human or clinical diagnosis.

About Abbkine Scientific Co. Ltd

Abbkine Scientific is a company founded by a team of scientists and marketing experts. Headquartered in China, the company has been able to provide innovative and effective scientific research products and tools to investigators, helping to accelerate studies in animal and human health.