Cleaved-Notch 2 (D1733) Polyclonal Antibody Review

The NOTCH2 gene provides instructions for making a protein called Notch2, a member of the Notch family of receptors. The Notch2 receptor has several major parts. A region of the receptor called the extracellular domain extends from the surface of the cell and binds to ligands. This binding triggers the part of the receptor inside the cell, known as the intracellular domain or NICD, to be cut (cleaved) from the rest of the protein. Research indicates that Notch2 signaling is important for the development of cells destined to be part of the heart, liver, kidneys, teeth, bones, and other structures in a growing embryo.

Abbkine Cleaved-Notch 2 (D1733) Polyclonal Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using synthesized peptide derived from the Internal region of human Notch 2. at AA rangle: 1660-1740. The antibody  was tested to apply in ELISA, IHC-p and WB with Human, Mouse and Rat samples. The suggested starting dilutions are: WB: 1:500-1:2000, IHC-p: 1:100-1:300, ELISA: 1:40000.

293 cell lysates were used. Western blot signals were detected using an Odyssey machine. A protein band which was assumed to be the cleaved Notch 2 protein was detected between 100-130KD using Cleaved-Notch 2 (D1733) (ABP50052) antibody at 1:500 dilution and a rabbit Dylight 680 secondary antibody (A23720). The antibody works in accordance with expectation.