Ki 67 Monoclonal Antibody Review

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. It is strictly associated with cell proliferation. During interphase, the Ki-67 antigen can be exclusively detected within the cell nucleus, whereas in mitosis most of the protein is relocated to the surface of the chromosomes. Ki-67 protein is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent from resting cells (G0).

Abbkine Ki 67 Monoclonal Antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. It recognizes endogenous levels of murine Ki-67 protein. It will also detect endogenous levels of human Ki-67 protein. The antibody has been shown to work in IHC-p and IF. Abbkine suggested the starting dilutions are as follows: IHC-p: 1:200, IF: 1:200.

Ki-67 is an excellent marker to determine the growth fraction of a given cell population. The fraction of Ki-67-positive tumor cells (the Ki-67 labeling index) is often correlated with the clinical course of cancer. We studied mouse testis tissue for Immunofluorescence analysis using Abbkine Ki 67 Monoclonal Antibody. Fortunately, the performance of the antibody was excellent, an immunopositive signal was found.