Selected primary antibody|TNF-α antibody recommendation

Review of Related Articles:

Research
Hotspots of Cell Apoptosis: Bcl-2 Monoclonal Antibody

Abbkine
P53 antibody helps basic cancer research!

Marker of myofibroblast antibody-α-SMA/ACTA2 Monoclonal Antibody

Tumor necrosis factor (TNF, cachexin, or
cachectin; once named as tumor necrosis factor alpha or TNFα) is a cell
signaling protein (cytokine) involved in systemic inflammation and is one of
the cytokines that make up the acute phase reaction. It is produced chiefly by
activated macrophages, although it can be produced by many other cell types
such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and
neurons. TNF is a member of the TNF superfamily, consisting of various
transmembrane proteins with a homologous TNF domain.

The primary role of TNF is in the regulation of immune cells. TNF, being an endogenous pyrogen, is able to induce fever, apoptotic cell death, cachexia, inflammation and to inhibit tumorigenesis and viral replication and respond to sepsis via IL1- & IL6-producing cells. Dysregulation of TNF production has been implicated in a variety of human diseases including Alzheimer's disease, cancer, major depression, psoriasis and inflammatory bowel disease (IBD). Though controversial, studies of depression and IBD are currently being linked to increased levels of TNF. Recombinant TNF is used as an immunostimulant under the INN tasonermin. TNF can be produced ectopically in the setting of malignancy and parallels parathyroid hormone both in causing secondary hypercalcemia and in the cancers with which excessive production is associated.

Fig: Anti-TNF-α therapies: the next generation (https://www.nature.com/articles/nrd1175)

Abbkine TNF-α
Polyclonal Antibody helps basic cancer research! Basic information about
the product:

Product name	#CAT	Reactivity	Applications	Dilution ratio	Observed Band(KD)
TNF-α Polyclonal Antibody	ABP0127	Human/Mouse/Rat	WB/IF/IHC-P/ELISA	WB (1:500-1:2000) IF (1:200-1:1000) IHC-P (1:100-1:300) ELISA（1/10000）	16KD

Abbkine TNF-α Polyclonal Antibody verification results:

Western Blot analysis using Abbkine TNF-α Polyclonal Antibody.

Immunofluorescence analysis of human stomach tissue using Abbkine TNF-α Polyclonal Antibody (1:200).

Immunohistochemical analysis of paraffin-embedded Human stomach (1:100).

Marker of myofibroblast antibody-α-SMA/ACTA2 Monoclonal Antibody

ACTA2 (actin alpha 2) is an actin protein
with several aliases including alpha-actin, alpha-actin-2, aortic smooth muscle
or alpha smooth muscle actin (α-SMA, SMactin, alpha-SM-actin, ASMA). Actins are
a family of globular multi-functional proteins that form microfilaments. ACTA2
is one of 6 different actin isoforms and is involved in the contractile
apparatus of smooth muscle. ACTA2 (as with all the actins) is extremely highly
conserved and found in nearly all mammals.

In humans, ACTA2 is encoded by the ACTA2
gene located on 10q22-q24. Mutations in this gene cause a variety of vascular
diseases, such as thoracic aortic disease, coronary artery disease, stroke,
Moyamoya disease, and multisystemic smooth muscle dysfunction syndrome.

ACTA2 (commonly referred to as alpha-smooth muscle actin or α-SMA) is often used as a marker of myofibroblast formation.

Figure: Diverse origins of the
myofibroblast—implications for kidney fibrosis (From Nature Reviews)

Abbkine α-SMA Monoclonal Antibody Helps Basic Cancer Research! Basic information about the product:

Product name	#CAT	Reactivity	Applications	Dilution ratio	Observed Band(KD)
α-SMA Monoclonal Antibody	ABM0052	Human/Mouse/ Rat	WB/IF/IHC-P	WB (1:10000-1:100000) IF (1:100-1:200) IHC-P (1:1000-1:2000)	42KD

Abbkine α-SMA Monoclonal Antibody verification results:

	Western blot analysis of 1) Hela, 2) 3T3, 3) rat brain using α-SMA Monoclonal Antibody.
	Immunofluorescence analysis of human liver tissue.
	Immunohistochemical analysis of paraffin-embedded mouse liver tissue

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