2020年1月15日星期三

Protein Quantification- Reliable and Stable Choice of Excellence

Accurate protein quantification is
necessary for protein-related experiments that involve research in molecular
biology, cell biology, biochemistry, developmental biology, and neuroscience.
Abbkine protein quantification products include Protein Quantification Kits
(BCA Assay and Bradford Assay), SuperLumia ECL HRP Substrate Kit, Protein Gel Flash
Staining Kit, Colorcode Prestained Protein Marker and so on.





Protein
Quantification Kits





Featured products:





1. Protein Quantification Kits (BCA Assay)





• Save time—Easier and 4x faster than
traditional Lowry methods.





• High sensitivity—The detection line is as low as 25ug/ml, and the
minimum detection protein is 0.5ug.





• Good compatibility—unaffected by most ionic and non-ionic
detergents. Compatible with up to 5% SDS, 5% Triton X-100, 5% Tween-20, 60, 80
in the sample.





• Excellent linearity-linear standard curve range: 50– 1000ug/ml.





• Good stability—Protein-to-protein differences are smaller compared
to dye-bound methods.





2. Protein Quantification Kits (Bradford Assay)





• Save time-rapid color development.





• Good compatibility—unaffected by most chemicals. The compatible
concentrations of mercaptoethanol and dithiothreitol in the samples can reach
1mM and 5mM, respectively.





• Good detection range—The detection range is 50-1000ug/ml.





ECL





SuperLumia ECL HRP Substrate Kit developed
by Abbkine is based on the classic peroxidase substrate system innovation and
optimization. It is used to enhance chemiluminescence (ECL) and can directly
replace other expensive ECL products without re-optimizing experimental
conditions. It is the perfect combination of superior quality and affinity
price.





Protein
Gel Flash Staining Kit





Protein staining can be detected in the
range of 10ng to 5μg, which only takes 0.5-1hours. It is about 5-10 times more sensitive than the
traditional Coomassie R-250 dye.









Fig1.
Various volumes of BSA samples were separated on 10% SDS-PAGE, stained with
protein gel flash staining working solution for 30 minutes.





Protein
Marker





Abbkine's Colorcode Prestained Protein Marker (10-180 kDa) contains 3 colors, totaling 10 protein standards (10, 17, 25, 33, 40, 53, 70, 95, 130, 180 kDa). The orange band is 70 kDa; the green band is 10 kDa. Thaw at room temperature. Mix gently and thoroughly to ensure a homogeneous solution.









Fig2. Image is from a 15% Tris-glycine gel (SDS-PAGE) transferred to membrane using Abbkine Colorcode Prestained Protein Marker (10-180 kDa).





Abbkine Protein Quantification product
recommendation:






Product name

#Cat

Szie

Price ($)

Protein Quantification Kit (BCA Assay)

KTD3001

500/2000/
5000T
45/100/180

Protein Quantification Kit (Bradford Assay)

KTD3002

500/2000/5000T

30/70/120

Protease Inhibitor Cocktail (100X)

BMP1001
1ml/1ml*5
40/120

SuperLumia ECL HRP Substrate Kit

K22020

30/100/200/500ml

30/75/140/280

SuperLumia ECL Plus HRP Substrate Kit

K22030

30/100/200/500ml
45/110/210/440

Protein Gel Flash Staining Kit

K21010
100/250/500ml
60/120/200

Colorcode Prestained Protein Marker (10-180
kDa)

BMM3001
100μl/250μl*2/250μl*20
20/62/496

Colorcode Prestained Protein Marker (15-130
kDa)

BMM3002
100μl/250μl*2/250μl*20
16/48/384

2020年1月8日星期三

Hard work, Do not forget your initiative mind-—Abbkine Breaks 1400 citations

Since
Abbkine document collection began, as of December 31, 2019, the number of
English articles published by google using Abbkine products has exceeded 1400,
with an impact factor exceeding 5400 points.





Thank you
for your trust and support to Abbkine. We will continuously stimulate our
internal creativity, provide competitive biomedical products and services, and
continuously create maximum value for our customers. With a view to becoming a
respected and world-class provider of biomedical products and services.





Figure 1:
Number of English Articles Published Using Abbkine Products from 2017 to 2019





In December 2019, Abbkine added 200+ citations. Some high-score citations are as below.





1.LECT2, a Ligand for Tie1, Plays a Crucial Role in Liver Fibrogenesis.





https://doi.org/10.1016/j.cell.2019.07.021





Magazine:
Cell





Impact: 24.38





Abstract:
Liver
fibrosis is a very common condition seen in millions of patients with various
liver diseases, and yet no effective treatments are available owing to poorly
characterized molecular pathogenesis. Here, we show that leukocyte cell-derived
chemotaxin 2 (LECT2) is a functional ligand of Tie1, a poorly characterized
endothelial cell (EC)-specific orphan receptor. Upon binding to Tie1, LECT2
interrupts Tie1/Tie2 heterodimerization, facilitates Tie2/Tie2
homodimerization, activates PPAR signaling, and inhibits the migration and tube
formations of EC. In vivo studies showed that LECT2 overexpression inhibits
portal angiogenesis, promotes sinusoid capillarization, and worsens fibrosis,
whereas these changes were reversed in Lect2-KO mice. Adeno-associated viral vector
serotype 9 (AAV9)-LECT2 small hairpin RNA (shRNA) treatment significantly
attenuates fibrosis. Upregulation of LECT2 is associated with advanced human
liver fibrosis staging. We concluded that targeting LECT2/Tie1 signaling may
represent a potential therapeutic target for liver fibrosis, and serum LECT2
level may be a potential biomarker for the screening and diagnosis of liver
fibrosis.





Products
using from Abbkine:





IPKine™ HRP, Goat Anti-Mouse IgG HCS
(CAT#: A25112)





IPKine™ HRP, Goat Anti-Mouse IgG LCS (CAT#: A25012)





2. EMS1 and BRI1 control separate biological processes via extracellular domain diversity and intracellular domain conservation.





https://www.nature.com/articles/s41467-019-12112-w





Magazine: Nature
Communications volume





Impact: 12.19





Abstract: In
flowering plants, EMS1 (Excess Microsporocytes 1) perceives TPD1 (Tapetum
Determinant 1) to specify tapeta, the last somatic cell layer nurturing pollen
development. However, the signaling components downstream of EMS1 are
relatively unknown. Here, we use a molecular complementation approach to
investigate the downstream components in EMS1 signaling. We show that the EMS1
intracellular domain is functionally interchangeable with that of the
brassinosteroid receptor BRI1 (Brassinosteroid Insensitive 1). Furthermore,
expressing EMS1 together with TPD1 in the BRI1 expression domain could
partially rescue bri1 phenotypes, and led to the dephosphorylation of BES1, a
hallmark of active BRI1 signaling. Conversely, expressing BRI1 in the EMS1
expression domain could partially rescue ems1 phenotypes. We further show that
PpEMS1 and PpTPD1 from the early land plant Physcomitrella patens could
completely rescue ems1 and tpd1 phenotypes, respectively. We propose that EMS1 and
BRI1 have evolved distinct extracellular domains to control different
biological processes but can act via a common intracellular signaling pathway.





Products using from Abbkine:





Anti-Plant Actin Mouse Monoclonal Antibody (3T3) (CAT#: A01050)





3. PLK4 deubiquitination by Spata2‐CYLD suppresses NEK7‐mediated NLRP3 inflammasome activation at the centrosome.





https://www.embopress.org/doi/abs/10.15252/embj.2019102201





Magazine: EMBO
JOURNAL





Impact: 10.55





Abstract:
The innate
immune sensor NLRP3 assembles an inflammasome complex with NEK7 and ASC to
activate caspase‐1 and drive the maturation of proinflammatory cytokines IL‐1β
and IL‐18. NLRP3 inflammasome activity must be tightly controlled, as its
over‐activation is involved in the pathogenesis of inflammatory diseases. Here,
we show that NLRP3 inflammasome activation is suppressed by a centrosomal
protein Spata2. Spata2 deficiency enhances NLRP3 inflammasome activity both in
the macrophages and in an animal model of peritonitis. Mechanistically, Spata2
recruits the deubiquitinase CYLD to the centrosome for deubiquitination of
polo‐like kinase 4 (PLK4), the master regulator of centrosome duplication.
Deubiquitination of PLK4 facilitates its binding to and phosphorylation of NEK7
at Ser204. NEK7 phosphorylation in turn attenuates NEK7 and NLRP3 interaction,
which is required for NLRP3 inflammasome activation. Pharmacological or
shRNA‐mediated inhibition of PLK4, or mutation of the NEK7 Ser204
phosphorylation site, augments NEK7 interaction with NLRP3 and causes increased
NLRP3 inflammasome activation. Our study unravels a novel centrosomal
regulatory pathway of inflammasome activation and may provide new therapeutic
targets for the treatment of NLRP3‐associated inflammatory diseases.





Products using from Abbkine:





IFKine™ Green Donkey Anti-Mouse IgG (CAT#: A24211)





4. Cross-Microbial Protection via Priming a Conserved Immune Co-Receptor through Juxtamembrane Phosphorylation in Plants





https://doi.org/10.1016/j.chom.2019.10.010





Magazine: Cell
Host & Microbe





Impact: 10.5





Abstract: Living
organisms can be primed for potentiated responses to recurring stresses based
on prior experience. However, the molecular basis of immune priming remains
elusive in plants that lack adaptive immunity. Here, we report that bacterial
challenges can prepare plants for fungal attacks by inducing juxtamembrane
phosphorylation of CERK1, the co-receptor indispensable for signaling in
response to the fungal elicitor chitin. This phosphorylation is mediated by
BAK1, a co-receptor for signaling in response to multiple elicitors. BAK1
interacts with CERK1, and loss of BAK1 reduces priming phosphorylation of
CERK1. Juxtamembrane phosphomimetic mutations of CERK1 confer accelerated
chitin responses and fortified fungal resistance without triggering
constitutive immunity, whereas juxtamembrane phosphodeficient mutations
diminish bacteria-induced protection against fungal infection. These findings
reveal that crosstalk between cell-surface immune co-receptors can prime
defense and demonstrate that juxtamembrane phosphorylation of plant
receptor-like kinases can occur independent of kinase activation to place the
protein into a prime state.





Products using from Abbkine:





Anti-GST Tag Mouse Monoclonal Antibody (2A8) (CAT#: A02030)





5. Extracellular vesicles of carcinoma-associated fibroblasts creates a pre-metastatic niche in the lung through activating fibroblasts





https://molecular-cancer.biomedcentral.com/articles/10.1186/s12943-019-1101-4





Magazine: Molecular
Cancer





Impact: 9.17





Abstract: Carcinoma-associated
fibroblasts (CAFs) have been known to promote cancer progression by modifying
the primary tumor microenvironment. We aimed to elucidate the intercellular
communication between CAFs and secondary organs in salivary adenoid cystic
carcinoma (SACC) metastasis.





Products using from Abbkine:





FITC, Goat Anti-Rabbit IgG (CAT#: A22120)





Dylight 488, Goat Anti-Rabbit IgG
(CAT#: A23220)





Dylight 549, Goat Anti-Rabbit IgG (CAT#: A23320)





Please learn more details from https://www.abbkine.com/publications/ .

2020年1月3日星期五

The third generation of fluorescent dye AbFluor™/highly water-soluble fluorescent dye

AbFluor™ dyes are a
series of highly water-soluble fluorescent dyes introduced by Abbkine after
many years of research, covering the ultraviolet, visible and near-infrared
spectrum, suitable for labeling biological macromolecules, especially proteins
and nucleic acids. Their excellent hydrophilicity enables protein-conjugated
labeling to be performed easily in aqueous media, while minimizing the need for
organic solvents. AbFluor™ dyes not only have better fluorescence performance
than other fluorescent dyes (such as FITC, TRITC, Alexa Fluor and Dylight
dyes), but also the coupling products are more stable.





Why recommend
AbFluor™ fluorescent dyes?





With innovative
modifications to the core structure of the dye, AbFluor™ dyes have more
brilliant properties than other commercial dyes, including stronger fluorescent
brightness, better light stability, wider pH tolerance range, and better
penetration and lower background. As a third-generation fluorescent dye,
AbFluor™ dyes not only perform better than traditional dyes (such as FITC,
TRITC, and Cy dyes), but also perform better than other second-generation
commercial dyes (such as Alexa Fluor, Dylight and IRDye).





1. AbFluor™ dyes
have excellent core structure, stable reactive groups, and good light
stability, ensuring high stability and high labeling efficiency of
bioconjugation.





2. High water
solubility (>100 mg/ml), which minimizes fluorescence quenching; it is also
easily soluble in other polar solvents, such as DMSO, DMF, methanol and
ethanol.





3. A wide range of
fluorescence spectrum, covering ultraviolet, visible, and near-infrared, to
meet most of the experimental needs of researchers.





4. Good pH
tolerance, stable in pH 2-11.





Taking AbFluor™ 488
as an example, the following is an IF comparison chart of different dye effects:









Different dye : FITC     Alexa Fluor 488       AbFluor™ 488





So, are you excited,
and what are you waiting for???





Abbkine AbFluor™ product recommendation:






Product name

#Cat

Szie

Price ($)

LinKine™ AbFluor™ 405 Labeling Kit

KTL0510

3*20μg/100μg/
3*100μg/1mg

1200/1400/
1600/2500

LinKine™ AbFluor™ 488 Labeling Kit

KTL0520

3*20μg/100μg/
3*100μg/1mg

1200/1400/
1600/2500

LinKine™ AbFluor™ 555 Labeling Kit

KTL0530

3*20μg/100μg/
3*100μg/1mg

1200/1400/
1600/2500

LinKine™ AbFluor™ 594 Labeling Kit

KTL0540

3*20μg/100μg/
3*100μg/1mg

1200/1400/
1600/2500

LinKine™ AbFluor™ 647 Labeling Kit

KTL0560

3*20μg/100μg/
3*100μg/1mg

1200/1400/
1600/2500

LinKine™ AbFluor™ 680 Labeling Kit

KTL0580

3*20μg/100μg/
3*100μg/1mg

1200/1400/
1600/2500

LinKine™ AbFluor™ 770 Labeling Kit

KTL0590

3*20μg/100μg/
3*100μg/1mg

1200/1400/
1600/2500