2020年1月15日星期三

Protein Quantification- Reliable and Stable Choice of Excellence

Accurate protein quantification is
necessary for protein-related experiments that involve research in molecular
biology, cell biology, biochemistry, developmental biology, and neuroscience.
Abbkine protein quantification products include Protein Quantification Kits
(BCA Assay and Bradford Assay), SuperLumia ECL HRP Substrate Kit, Protein Gel Flash
Staining Kit, Colorcode Prestained Protein Marker and so on.





Protein
Quantification Kits





Featured products:





1. Protein Quantification Kits (BCA Assay)





• Save time—Easier and 4x faster than
traditional Lowry methods.





• High sensitivity—The detection line is as low as 25ug/ml, and the
minimum detection protein is 0.5ug.





• Good compatibility—unaffected by most ionic and non-ionic
detergents. Compatible with up to 5% SDS, 5% Triton X-100, 5% Tween-20, 60, 80
in the sample.





• Excellent linearity-linear standard curve range: 50– 1000ug/ml.





• Good stability—Protein-to-protein differences are smaller compared
to dye-bound methods.





2. Protein Quantification Kits (Bradford Assay)





• Save time-rapid color development.





• Good compatibility—unaffected by most chemicals. The compatible
concentrations of mercaptoethanol and dithiothreitol in the samples can reach
1mM and 5mM, respectively.





• Good detection range—The detection range is 50-1000ug/ml.





ECL





SuperLumia ECL HRP Substrate Kit developed
by Abbkine is based on the classic peroxidase substrate system innovation and
optimization. It is used to enhance chemiluminescence (ECL) and can directly
replace other expensive ECL products without re-optimizing experimental
conditions. It is the perfect combination of superior quality and affinity
price.





Protein
Gel Flash Staining Kit





Protein staining can be detected in the
range of 10ng to 5μg, which only takes 0.5-1hours. It is about 5-10 times more sensitive than the
traditional Coomassie R-250 dye.









Fig1.
Various volumes of BSA samples were separated on 10% SDS-PAGE, stained with
protein gel flash staining working solution for 30 minutes.





Protein
Marker





Abbkine's Colorcode Prestained Protein Marker (10-180 kDa) contains 3 colors, totaling 10 protein standards (10, 17, 25, 33, 40, 53, 70, 95, 130, 180 kDa). The orange band is 70 kDa; the green band is 10 kDa. Thaw at room temperature. Mix gently and thoroughly to ensure a homogeneous solution.









Fig2. Image is from a 15% Tris-glycine gel (SDS-PAGE) transferred to membrane using Abbkine Colorcode Prestained Protein Marker (10-180 kDa).





Abbkine Protein Quantification product
recommendation:






Product name

#Cat

Szie

Price ($)

Protein Quantification Kit (BCA Assay)

KTD3001

500/2000/
5000T
45/100/180

Protein Quantification Kit (Bradford Assay)

KTD3002

500/2000/5000T

30/70/120

Protease Inhibitor Cocktail (100X)

BMP1001
1ml/1ml*5
40/120

SuperLumia ECL HRP Substrate Kit

K22020

30/100/200/500ml

30/75/140/280

SuperLumia ECL Plus HRP Substrate Kit

K22030

30/100/200/500ml
45/110/210/440

Protein Gel Flash Staining Kit

K21010
100/250/500ml
60/120/200

Colorcode Prestained Protein Marker (10-180
kDa)

BMM3001
100μl/250μl*2/250μl*20
20/62/496

Colorcode Prestained Protein Marker (15-130
kDa)

BMM3002
100μl/250μl*2/250μl*20
16/48/384

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