New product: IL-6, TNF-alpha, bFGF, IFN-gamma recombinant protein upgrading!

The new product line in this recombinant protein series:

Human
IL-6 protein：Interleukin 6 (IL-6) is a pleiotropic
cytokine that plays an important role in host defense by regulating immune and
inflammatory responses. IL-6 is an important member of the cytokine network and
is central to the acute inflammatory response. After the production of IL-6, it
induces the production of C-reactive protein (CRP) and procalcitonin (PCT),
which are associated with inflammatory diseases and infections. The degree is
directly related. IL-6 can quickly diagnose early inflammation and warn of
sepsis.

Human
TNF-alpha protein：Tumor necrosis factor-α (TNF-α) is a cytokine
involved in systemic inflammation, mainly secreted by macrophages. Its main function
is to regulate immune cells. As an endogenous pyrogen, it can cause fever,
cause apoptosis, prevent tumorigenesis and virus replication. Its dysfunction
is thought to be related to many diseases, such as Alzheimer's disease,
psoriasis, cancer, major depression, and enteritis. TNF-a is an important part
of the cell signaling pathway, so it has become the target of multiple drugs.

Human
bFGF protein、Mouse bFGF protein：

Basic fibroblast growth factor (bFGF) is a trace
substance present in mammals and a multifunctional cell growth factor. bFGF has
obvious tendency activity to wound cells, induces inflammatory cells,
fibroblasts, vascular endothelial cells to move to the wound site, activates
the phagocytic function of macrophages, improves the body's immune activity,
and significantly reduces the chance of wound infection.

Human
IFN-gamma protein, His Tag、Mouse IFN-gamma protein, His Tag：IFN-gamma belongs
to the type II interferon family， which is
widely involved in immune and inflammatory responses. Proper activation of IFN-γ signaling pathway can promote the activation
of macrophages and mediate the host's defense function against pathogens. At
the same time, IFN-γ signaling
pathway can also play an immunomodulatory role in anti-tumor immunity.

New product details:

Product supply form: freeze-dried powder
form, very stable at room temperature. Recommendation: Lyophilized protein
product should be stored desiccated below -20°C.. After dissolution, the
product can be stored at 4℃ for 2-7 days, please keep it below -20℃ for long-term
use. For long-term storage, it is recommended to add carrier protein (0.1% HSA
or BSA), and store it separately as appropriate to avoid repeated freezing and
thawing.

Application areas: antibody preparation or
detection, ELISA standards, cell or tissue culture and other functional
studies.

Price guarantee: better discounts, lower prices.

Product name	CAT#	Preparation method	Purity	Formulation	Size/ Price	Fig. SDS-PAGE
Human IL-6 protein	PRP1012	E. coli	> 95 %	Lyophilized powder	5ug/$48 20ug/$120 100ug/$300 1mg/$800
Human TNF-alpha protein	PRP1013	E. coli	> 95 %	Lyophilized powder	10ug/$48 50ug/$120 100ug/$170 1mg/$800
Human bFGF protein	PRP1010	E. coli	> 95 %	Lyophilized powder	10ug/$48 50ug/$120 100ug/$170 1mg/$900
Mouse bFGF protein	PRP1011	E. coli	> 95 %	Lyophilized powder	10ug/$48 50ug/$120 100ug/$170 1mg/$900
Human IFN-gamma protein, His Tag	PRP1014	E. coli	> 95 %	Lyophilized powder	20ug/$48 100ug/$120 500ug/$450 1mg/$800
Mouse IFN-gamma protein, His Tag	PRP1015	E. coli	> 95 %	Lyophilized powder	20ug/$48 100ug/$120 500ug/$450 1mg/$800

Abbkine focuses on the fields of immunology
and cytology, and is committed to innovating and developing various types of
antibodies, proteins, analytical reagents and kits, with a view to becoming a
key promoter in the fields of life science research and development, and drug
development. Here, we present to you the favorite products of protein and
immune research users, from basic immunological products, such as protein
extraction and quantification, to internal reference label antibodies, primary
and secondary antibodies for immunological experiments; cell research users’
favorite Products ranging from dyes and kits for cell state detection,
organelle extraction kits, cell substructure staining and cell metabolism
detection products, to cytokine and protein detection kits for cell culture,
just to help you Research career!

About Abbkine Scientific Co., Ltd.

Abbkine Scientific Co., Ltd. was founded in
2012. Our mission is to become a respected and world-class supplier of
biomedical products and services. Through a clear core strategy and a corporate culture that
promotes learning, innovation, cooperation, and excellence, we will stimulate
our inherent creativity, provide competitive biomedical products and services,
and continue to create maximum value for customers and achieve our mission.

How to choose appropriate Catalase (CAT) Activity Assay Kit?

Catalase (CAT) is an antioxidant enzyme commonly found in almost all living organisms. Its main function is to catalyze the decomposition of hydrogen peroxide into water and oxygen and remove hydrogen peroxide in the body, thus preventing cells from being poisoned by H₂O₂. It is one of the key enzymes in the biological defense system and provides an antioxidant defense mechanism for the body.

In this article, for CAT Activity Assay Kit, the author chose different brands (Cayman Chemical, Abcam, Biovision) and compared them with Abbkine CheKine™ Catalase (CAT) Activity Assay Kit (Cat#: KTB1040) from several aspects such as assay principle, detection, sample type and size/price.

Summary: For CAT Activity Assay Kit, the brand Abbkine and Cayman Chemical are the similar in Assay Principle. Methanol is taken as a reaction substrate. While Abcam and Biovision take H₂O₂ as a reaction substrate.

But H₂O₂ is not only a single substrate of catalase (CAT), other peroxidases in the sample may interfere with the experimental results, and the standard curve error may be caused by instability of hydrogen peroxide. While methanol is the unique substrate of catalase, other peroxidases cannot use methanol as substrate, so interference signals are avoided.

Details of different brands of products are as follows,

Brand	Assay Principle	Sample type	Size/Price
Abbkine	This assay kit utilizes the peroxidatic function of catalase for measuring catalase activity, based on the reaction of catalase with methanol, with the presence of an optimal concentration of H2O2. The formaldehyde produced can be measured colorimetrically at OD 540 nm. Therefore, the catalase activity present in the sample is proportional to the signal obtained.	Tissue Homogenate, Cell Lysate, serum, plasma, erythrocyte lysate	48T/$40 96T/$60 480T/$180
Cayman Chemical	This assay kit utilizes the peroxidatic function of catalase for measuring catalase activity, based on the reaction of catalase with methanol, with the presence of an optimal concentration of H2O2. The formaldehyde produced can be measured colorimetrically at OD 540 nm. Therefore, the catalase activity present in the sample is proportional to the signal obtained.	Tissue Homogenate, Cell Lysate, serum, plasma, erythrocyte lysate	96T/$270 480T/$490
Abcam	The catalase present in the sample reacts with hydrogen peroxide (H2O2) to produce water and oxygen. The unconverted H2O2 reacts with probe to produce a product that can be measured colorimetrically at OD 570 nm.	Serum, Plasma, Cell Lysate, Tissue Lysate, Urine	100T/$415
Biovision	The catalase present in the sample reacts with hydrogen peroxide (H2O2) to produce water and oxygen. The unconverted H2O2 reacts with probe to produce a product that can be measured colorimetrically at OD 570 nm.	Tissue Homogenate, Cell Lysate, erythrocyte lysate	100T/$340

About Abbkine Scientific Co., Ltd.

Abbkine serves global scientists in the field of proteomics and cytology and is committed to the innovation and development of various scientific reagents related to proteomics and cytology, expecting to accelerate the pace of life science research and drug discovery. Proteomics products cover the preparation of samples (protein extraction, purification, coupling), protein quantification, antibodies and kits for protein detection. Cytology products involve cytokines (cell culture), cell status detection, cell staining, organelle extraction, cell metabolism and cytopathology reagents (kits). Abbkine relies on the product portfolio and unique marketing support as the main market strategy and product innovation mode, with ultimate aim to facilitate your research career.

How to choose appropriate NADP/NADPH Assay Kit?

NADP (coenzyme II) is the coenzyme of many redox reactions, including NADP+ (oxidation type) and NADPH (reduction type). NADP+ is also involved in biosynthesis reactions, such as lipid and nucleic acid synthesis. In animal cells, the oxidation stage of pentose phosphate pathway (PPP) is the main source of NADPH. 

In this article, for NADP/NADPH Assay Kit, the author chose different brands (BioAssay Systems, Abcam, Biovision) and compared them with Abbkine CheKine™NADP/NADPH Assay Kit (Cat#: KTB1010) from several aspects such as assay principle, detection, sample type and size/price.

Summary:

These brands are the similar in Assay Principle, Detection and Sample type. Prices are different. The price of Abbkine NADP/NADPH Assay Kit (Cat#: KTB1010) is the lowest.

Besides good price, Abbkine NADP/NADPH Assay Kit (Cat#: KTB1010) has other advantages. The detection limit of Abbkine NADP/NADPH Assay Kit is 0.1 µM and linearity up to 4 µM NADP+/NADPH in 96-well plate assay.

Fig. Standard Curve of NADPH in 96-well plate assay. The y-axis is ΔOD and the x-axis is NADPH concentration (uM).

Details of different brands for NADP/NADPH Assay Kit are as follows,

Brand	Assay Principle	Detection	Sample type	Size/Price
Abbkine	The assay is based on an enzymatic cycling reaction in which NADP+ is reduced to NADPH. NADPH reacts with a colorimetric probe that produces a colored product which can be measured at 565 nm.	Detects NADP+, NADPH, or total NADP+/NADPH	Tissue Extracts, Cell Lysate	48T/$90 96T/$150
BioAssay Systems	The assay is based on an enzymatic cycling reaction in which NADP+ is reduced to NADPH. NADPH reacts with a colorimetric probe that produces a colored product which can be measured at 565 nm.	Detects NADP+, NADPH, or total NADP+/NADPH	Tissue Extracts, Cell Lysate	100T/$379
Abcam	The assay is based on an enzymatic cycling reaction in which NADP+ is reduced to NADPH. NADPH reacts with a colorimetric probe that produces a colored product which can be measured at 450 nm.	Detects NADP+, NADPH, or total NADP+/NADPH	Tissue Extracts, Cell Lysate	100T/$615
Biovision	The assay is based on an enzymatic cycling reaction in which NADP+ is reduced to NADPH. NADPH reacts with a colorimetric probe that produces a colored product which can be measured at 450 nm.	Detects NADP+, NADPH, or total NADP+/NADPH	Tissue Extracts, Cell Lysate	100T/$535

About Abbkine Scientific Co., Ltd.

Abbkine serves global scientists in the field of proteomics and cytology and is committed to the innovation and development of various scientific reagents related to proteomics and cytology, expecting to accelerate the pace of life science research and drug discovery. Proteomics products cover the preparation of samples (protein extraction, purification, coupling), protein quantification, antibodies and kits for protein detection. Cytology products involve cytokines (cell culture), cell status detection, cell staining, organelle extraction, cell metabolism and cytopathology reagents (kits). Abbkine relies on the product portfolio and unique marketing support as the main market strategy and product innovation mode, with ultimate aim to facilitate your research career.

The Statement of Strengthening Epidemic Prevention for COVID-19

Dear Colleagues,

The situation for COVID-19’s outbreak in Wuhan is getting improved in the strong supports of our government and caring people all over the world now. We are very lucky that we all are safe at present, while it’s still very important for us to do well protections. We determine to do below provisional measures during the resumption of production until the end of the outbreak in order to ensure the safe of all colleagues and our clients strictly.

1. The establishment of epidemic prevention group, their works contain procurement of protection materials, temperature detection, virus kill, daily patrol and consults ect;
2. Purchasing a batch of substance such as medicine alcohol, chlorine disinfectant, medical surgical masks, protective clothing, temperature guns for epidemic prevention;
3. The offices, production laboratories, warehouses must be disinfected fully after daily work, and all windows must be opened;
4. The temperature detection is needed before and after daily work for everyone, any person has the temperature over 37.3℃ must be sent to the hospital immediately. Early detection, early isolation, early treatment;
5. All colleagues must wear mask and the colleagues in production department also must wear protective clothing, gloves, wash and change them frequently;
6. The new purchased materials must be kept in isolated room first, and transferred into warehouses or production rooms after being thoroughly sprayed and disinfected with 75% medicine alcohol;
7. The high temperature resistant consumables, such as bottle, pipe, experimental spear, must be sterilized via high heat and pressure, and those consumables don’t resistant to high temperature, such as box, bag, must be thoroughly sprayed and disinfected with 75% medicine alcohol to ensure the safe of products during the production;
8. During the process of packing box, the inner and outer surface of the box must be sprayed and disinfected with 75% medicine alcohol to ensure the packaging safety;
9. Cargos should be transported via reliable express company such as S.F. Express, FedEx, DHL to ensure the transportation safety;

Abbkine Scientific Co.,Ltd.

20^th, February 2020

How to Select an Antibody&Protein Coupling Kit with Super Cost Performance

1, The common definition of coupling technology

Enzymes, biotin, fluorescent dye, agarose, magnetic beads and other substances which are easy to detect or separate are covalently labeled or conjugated to antibodies, proteins or other small molecular substances by a certain technology.

2, Background of Coupling Technology

Coupling of antibodies, proteins or other small molecules is a necessary step in many types of biological experiments.

For example, in order to detect specific proteins in immunoassays (such as WB/IHC/IF and other immunological experiments), we couple primary antibody or secondary antibody with enzyme, fluorescent dye, etc.

In cell staining (biological imaging) applications, reactive dyes (fluorescent dye) are directly combined with extracellular or intracellular proteins (polypeptides) to produce visible and measurable staining.

Coupling agarose or magnetic beads to proteins or antibodies provides a targeted purification method. For example, the agarose (magnetic beads) coupled antibody used by Abbkine in IP experiments directly saves the use of Protein A/G.

3, Major Disclosure of Coupling Kits of Various Brands (Taking HRP Coupling Kits and Green Fluorescence Coupling Kits as Examples)

1. HRP Coupling Kits

Brand	Sample types	Contents of the kit	Duration of experiment	Size/Price
Abbkine	Samples contain free amino group	1. Activated HRP conjugates solution 2. HRP labeling solution 3. HRP quencher	3.5-4h	3*20ug/$100 100ug/$120 3*100ug/$140 1mg/$220
Abcam	Samples contain free amino group	1. Activated HRP conjugates solution 2. HRP labeling solution 3. HRP quencher	3.5-4h	30ug/￥$270 100ug/$275 300ug/$330 1mg/$555 5mg/$1887
ThermoFisher	Samples contain free amino group	1. Activated HRP conjugates solution 2. HRP labeling solution 3. HRP quencher 4. Protein A/G Column	overnight+＞2h	300µg/$652

* How does Abbkine HRP coupling kit remove free HRP (molecular weight 40KD) from binding antibody?

Abbkine HRP coupling kit was designed with this in mind. The kit can provide a low level of free HRP at the end of the reaction. Therefore, no filtration step is required, and the termination solution (quencher) provided in the kit will block the reactive groups of free HRP.

* Protein A/G column in Thermofisher brand is used to remove free HRP.

• Green fluorescence Coupling Kits 

Brand	Sample types	Contents of the kit	Duration of experiment	Size/Price
Abbkine	Samples contain free amino group	1. Activated Green fluorescence conjugates solution 2. labeling solution 3. Purification Column	1.5h	3*20ug/$120 100ug/$140 3*100ug/$160 1mg/$250
Abcam	Samples contain free amino group	1. Activated Green fluorescence conjugates solution 2. labeling solution 3. quencher	0.5-1h	30ug/$265 100ug/$266 300ug/$415 1mg/$645
ThermoFisher	Samples contain free amino group	1. Activated Green fluorescence conjugates solution 2. labeling solution 3. Purification Column	1.5-2h	100µg/$538

* The purpose of the purification column is to separate the unconjugated dye from the coupled product, because the dye is small molecules (molecular weight is about 1KD), and the purification column volume provided by Abbkine is 50KDa, 0.5 ml. The purification column provided by ThermoFisher is also aimed at the purification of antibody (the molecular weight of antibody is about 160KD). If the molecular weight of the protein purified by customers is small or large, they need to equip themselves with appropriate ultrafiltration tubes.

The following recommendations are made for the allocation of ultrafiltration tubes:

The molecular weight of the sample	Ultrafiltration tubes
6<MW<20K	3K
20<MW<60K	10K
60<MW<100K	30K
100<MW<200K	50K
200K<MW	100K

To sum up, which brand of coupling kit would you choose? As the fans of Abbkine, I'd like to summarize the characteristics and advantages of Abbkine LinKine™ products:

• Optimized Scheme-Follow the standard experimental procedure and obtain the best activity or fluorescence with excellent pre-activated dye, protein ratio and patented solution formula.
• The operation is simple and convenient-the operation can be realized in three steps, and the coupling group is bound to a primary amine site of an antibody or other protein.
• Customization-changing molar ratio, reaction buffer, pH value and other parameters can realize different levels of coupling and activity.
• High Efficiency Purification-The kit includes a purification column to ensure rapid and effective removal of unreacted dyes and excellent protein/antibody recovery.

Buy Abbkine selected primary antibodies, get high quality secondary antibodies

Abbkine's selected primary antibodies are the first choice for reliable trust, featuring a selection of popular research targets, strict quality control and quality assurance. We are grateful for everyone's recognition of this product line. In order to allow more customers to use good products and send out high-score articles, we are now launching the activity of buying selected primary antibodies and get high quality secondary antibodies.

Promotion time: From now to December 31^th, 2020

Details of activities:

As long as you buy selected primary antibodies, you can get designated high quality secondary antibodies (size: 100ul) for free or at a very preferential price. Designated secondary antibodies are as follows,

Product name	Cat#	Citation	Size	List Price($)	Preferential Price($)
HRP, Goat Anti-Mouse IgG	A21010	131	100ul	25	0
HRP, Goat Anti-Rabbit IgG	A21020	147	100ul	25	0
DyLight 488, Goat Anti-Mouse IgG	A23210	79	100ul	40	15
Dylight 488, Goat Anti-Rabbit IgG	A23220	107	100ul	40	15
Dylight 549, Goat Anti-Mouse IgG	A23310	24	100ul	40	15
Dylight 549, Goat Anti-Rabbit IgG	A23320	59	100ul	40	15
Dylight 594, Goat Anti-Mouse IgG	A23410	30	100ul	40	15
Dylight 594, Goat Anti-Rabbit IgG	A23420	46	100ul	40	15
Dylight 649, Goat Anti-Mouse IgG	A23610	13	100ul	40	15
Dylight 649, Goat Anti-Rabbit IgG	A23620	18	100ul	40	15
Dylight 800, Goat Anti-Mouse IgG	A23910	4	100ul	40	15
Dylight 800, Goat Anti-Rabbit IgG	A23920	12	100ul	40	15
IFKine™ Green Donkey Anti-Mouse IgG	A24211	11	100ul	70	20
IFKine™ Green Donkey Anti-Rabbit IgG	A24221	12	100ul	70	20
IFKine™ Red Donkey Anti-Mouse IgG	A24411	5	100ul	70	20
IFKine™ Red Donkey Anti-Rabbit IgG	A24421	22	100ul	70	20
IPKine™ HRP, Goat Anti-Mouse IgG LCS	A25012	13	100ul	70	20
IPKine™ HRP, Mouse Anti-Rabbit IgG LCS	A25022	9	100ul	70	20
IPKine™ HRP, Goat Anti-Mouse IgG HCS	A25112	8	100ul	70	20
IPKine™ HRP, Goat Anti-Rabbit IgG HCS	A25222	7	100ul	70	20

* IFKine™antibodies: IFKine™ are series of specially optimized secondary antibodies with improved brightness, photostability and less nonspecific hybridization and background. Donkey host and other species of serum/IgG absorbed make it ideal choice for fluorescence staining, especially in fluorescence multiple labelling.

*IPKine™ antibodies: 25 kD or 50 kD protein detection on Western blots after Immunoprecipitation is often suffered from heavy or light chain blotting contamination. Abbkine’s  IPKine™ products could solve these problems and bring you satisfying results with good performance.

Wuhan Institute of Virology Reveals New Coronavirus (2019-nCoV) may Bat Origin

On February 3, the Wuhan virus research institute of the Chinese academy of sciences/biosafety research center, Wuhan jinyintan hospital and Hubei provincial center for disease prevention and control cooperated in the identification and research of 2019 new coronavirus (2019-nCoV), which caused the Wuhan pneumonia epidemic, and published it online under the topic "a pneumonia outbreak associated with a new coronavirus of disease origin" (the link of the paper is https://www.nature.com/articles/s41586-020-2012-7). Relevant research teams revealed the basic biological characteristics of the coronavirus from nucleic acid detection, serological diagnosis, virus isolation and receptor utilization, providing important clues for epidemic control and drug research and development.

The research team obtained the whole genome sequence of the virus from the early samples of 5 patients. The similarity of the virus sequence from 5 patients reached 99.9%, and the sequence consistency with SARS-CoV was 79.5%. The amino acid analysis of virus conserved protein shows that 2019-nCoV and SARS-CoV belong to SARS-related coronavirus (SARSR-COV). The author further compared 2019-nCoV genome with some gene sequences of coronavirus detected early in the laboratory, and found that the virus is similar to the gene of a coronavirus (TG13 for short) derived from a bat sample. After sequencing the bat sample, the whole genome sequence of bat virus TG13 was obtained, and the sequence consistency of the two viruses was found as high as 96% (Figure 1).

Fig. 1. high-throughput sequencing obtains the whole genome sequence (A) of the virus from a patient sample; Sketch map of 2019-nCoV genome (b); Homology analysis with human SARS virus and bat SARSr-CoV (c); Evolutionary analysis using 2019-nCoV replicase gene (d)

The research team carried out nucleic acid detection on samples of bronchoalveolar lavage fluid (BALF), throat swab (OS) and other samples of patients in different periods, and found that the viral nucleic acid of early samples was about 1000 times higher than that of late samples. By using SARSr-CoV antigen stored in the laboratory at an early stage, they tested the patient's serum samples and found that the patient produced corresponding IgM and IgG antibodies (fig. 2). Subsequently, 2019-nCoV virus was isolated from bronchoalveolar lavage fluid of a critically ill patient, and its intracellularly clear coronavirus particle morphology was observed by electron microscopy (fig. 3).

Fig. 2. virus nucleic acid detection in seven patients (a); Serological changes in a patient (B); Detection of Antibody Level in Seven Patients (C)

Fig. 3. electron microscopic observation of 2019-ncov in cells

The research team found that 2019-nCoV can infect non-sensitive cells that express human ACE2 (angiotensin converting enzyme, cell receptor of SARS-CoV), indicating that 2019-nCoV can invade cells using receptor of SARS-CoV (fig. 4).

Fig. 4. 2019-nCoV infection in HeLa cells expressing ACE2 from human, bat, pig, civet and mouse

In the race against time and epidemic situation, the study provided more detailed basic biological characteristics of 2019-nCoV virus in a short period of time, and revealed that the natural host of the virus may be bats. The study also showed that 2019-nCoV can infect cells with the receptor of SARS-CoV, laying a working foundation for subsequent epidemic prevention and drug research.

Product Recommendation:

1. ACE2 Polyclonal Antibody(ABP56008)
2. DyLight 488, Goat Anti-Mouse IgG (A23210)
3. Dylight 488, Goat Anti-Rabbit IgG (A23220)
4. Dylight 549, Goat Anti-Mouse IgG (A23310)
5. Dylight 549, Goat Anti-Rabbit IgG (A23320)
6. Dylight 594, Goat Anti-Mouse IgG (A23410)
7. Dylight 594, Goat Anti-Rabbit IgG (A23420)