Wuhan, China. 430074. 18th March 2017. Endotoxin is a membrane component of gram-negative bacteria also known as the lipopolysaccharides (LPS) strongly influence the transfection of DNA into sensitive cultured cells, primary cells. Increased endotoxins level leads to the reduction of transfection efficiencies. A single cell contains about 2 million LPS molecules, during cell lysis, the molecules are released. The work of the endotoxins is to imitate a strong immune response resulting in systemic inflammatory response syndrome or sepsis.Many commercial endotoxin removal kits are based upon non-affinity chromatography methods such as phase separation or the Ion exchange chromatography. These methods are inefficient at removing contaminated endotoxin residual from advance biotherapy products and they are also expensive and time-consuming.
Polymyxin B that can be used to neutralize the biological activities of endotoxin. Abbkine Scientific Company has come up with a endotoxin removal kit that helps in reducing endotoxin levels by 99% and all protein samples, but at the same time maximizing protein recovery. The resin consists of 4% Agarose cross-linked beads to which the polymyxin B has been coupled.
The PurKine™ Endotoxin removal kit is designed to achieve quality separation from gravity flow column, and each kit is standardized for high-grade clearance of endotoxins from antibodies, viral vectors and recombinant proteins. The PurKine™ Endotoxin removal kit enables quick processing of a large number of samples with high solute recovery, negligible hold-up volume, and minimal nonspecific absorption losses. The features and benefits of using endotoxin removal resin or the polymyxin B from PurKine™ include:
- Rapid: the polymyxin B allows multiple purifications. This helps in rapid screening and development methods. It also decreases the time used in every step associated with affinity methods.
- Effective: the kits offer a standardized method for endotoxin removal from protein and monoclonal antibodies, meaning it offers high capture efficiency and high reproducibility.
- Convenient: the technology used allows minimal manual intervention which makes it ideally suited where price sensitivity and current user requirements demand regeneration of affinity matrix.
- Flexibility: different pack sizes and has bulk resin, columns, and complete kits formats.
Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California, USA in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.
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