HER2 Monoclonal Antibody Review

The ErbB2 (HER2) proto-oncogene encodes a 185 kDa transmembrane, receptor-like glycoprotein with intrinsic tyrosine kinase activity. Amplification of the ErbB2 gene and overexpression of its product are detected in almost 40% of human breast cancers. ErbB2 is a key therapeutic target in the treatment of breast cancer and other carcinomas and targeting the regulation of ErbB2 degradation by the c-Cbl-regulated proteolytic pathway is one potential therapeutic strategy.

Abbkine HER2 Monoclonal Antibody are produced by immunizing mouse with a synthetic peptide. The isotype of the antibody is mouse IgG1. HER2 Monoclonal Antibody detects endogenous levels of total HER2 protein. The antibody does not cross-react with related kinases. Test confirmed the antibody can applied in WB, IF and IHC-p with Human, Mouse and Rat samples. Abbkine suggested the dilutions are: WB: 1:2000-4000, IHC: 1:200 and IF 1:200.

I purchased Abbkine HER2 Monoclonal Antibody for  immunofluorescence analysis of Rat-spleen tissue. I dilute the antibody according to the recommendation which is 1:200 for 4°C, overnight. Cy3 labled secondary antibody was diluted at 1:300 at room temperature for 50min. I show my picture in the left . I am very satisfied with this product-high quality research product with affordable price.