Western Blot Product Portfolio- Sample Preparation

Overview of western blot (WB):

Western Blot is an experimental method
commonly used in molecular biology, biochemistry and immunogenetics. The basic
principle is to color the cell or biological tissue samples treated by gel
electrophoresis through specific antibodies. Information on the expression of
specific proteins in the analyzed cells or tissues is obtained by analyzing the
staining position and staining depth.

Sample preparation:

Good protein is half the success of WB. We
need to lyse cells and tissues to extract the target protein. Once cracking
occurs, hydrolysis, dephosphorylation and denaturation begin. If appropriate
protease or phosphatase inhibitor is added to the cracking solution, these
reactions will be greatly slowed down and the chance of extracting good protein
will be increased.

It takes time and effort to prepare lysis
solution by oneself, and the whole experiment may be wasted due to improper
preparation. Why not try the protein extraction kit that Abbkine has packed for
you? The kit is equipped with optimized lysis components. You only need to
carry out the experiment according to the experimental steps.

Abbkine sample
preparation product recommendation:

Product name	#Cat	Szie	Price ($)
ExKine™ Nuclear and Cytoplasmic Protein Extraction Kit	KTP3001	50/200T	90/220
ExKine™ Nuclear Protein Extraction Kit	KTP3002	50/200T	80/200
ExKine™ Cytoplasmic Protein Extraction Kit	KTP3003	50/200T	80/200
ExKine™ Total Membrane Protein Extraction Kit	KTP3004	50/200T	80/200
ExKine™ Membrane and Cytoplasmic Protein Extraction Kit	KTP3005	50/200T	90/220
ExKine™ Total Protein Extraction Kit	KTP3006	50/200T	80/200
Protease Inhibitor Cocktail (100X)	BMP1001	1ml/1mlx5	40/120

The basic process of protein extraction

Characteristics and Advantages of Abbkine
Protein Extraction Kit:

• High purity and no pollution-the extracted protein has high purity and maintains natural activity, reducing cross contamination;
• Strong timeliness-non-denatured active protein can be purified within two hours;
• Compatible application-the extracted protein can be directly used in subsequent proteomics applications;
• Follow-up worry-free-kits own a special protease inhibitor package, anti-degradation, anti-useless;
• High cost performance.

Characteristics and Advantages of Abbkine Protease Inhibitor
Cocktail (100X):

Abbkine Protease Inhibitor Cocktail is an
universal protease inhibitor cocktail contains individual components, including
AEBSF, Aprotinin, Bestatin, E-64, Leupeptin and Pepstatin A with a broad
specificity for cysteine, serine, acid proteases, and aminopeptidases. This
protease inhibitor cocktail has been optimized and tested for mammalian cells
and tissue extracts.

You deserve to have good sample preparation
products!

Abbkine Holiday Arrangement of 2020 Spring Festival

Dear partners:

Thank you all for giving our
company full support in 2019. With the approaching of 2020 Spring Festival,
here Abbkine makes the following holiday arrangement:

Holiday Time: 20^th
Jan. 2020-31^th Jan. 2020 (12 Days).

Holiday Time for the
logistics department: 18^th Jan. 2020-2^nd Feb. 2020 (16
Days).

Sorry for the inconvenience
caused by the holiday. My sincere regards.

Abbkine Scientific Inc

23^th Dec.2019

Selected primary antibody| GSK3β Mouse Monoclonal antibody recommendation

Review of Related Articles:

Research
Hotspots of Cell Apoptosis: Bcl-2 Monoclonal Antibody

Abbkine
P53 antibody helps basic cancer research!

Marker
of myofibroblast antibody-α-SMA/ACTA2 Monoclonal Antibody

Selected
primary antibody|TNF-α antibody recommendation

Glycogen synthase kinase-3 (GSK-3) is a
serine-threonine kinase that has been evolutionarily very conservative.The
protein encoded by GSK3β (glycogen synthase kinase 3 beta) is a
serine-threonine kinase, belonging to the glycogen synthase kinase subfamily.
It is involved in energy metabolism, neuronal cell development, and body
pattern formation.

GSK-3β can also act on many signaling
protein structural proteins and transcription factors, and regulate cell
differentiation, proliferation, survival and apoptosis. GSK-3β is a key enzyme
involved in liver glucose metabolism. It inhibits its activity by
phosphorylating GS, reduces liver glucose synthesis, increases blood glucose
concentration in the body, is controlled by insulin in the insulin signaling
pathway, and participates in liver glucose metabolism.

Polymorphisms in GSK3β have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of GSK3β may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for GSK3β .

The picture is from https://link.springer.com/article/10.1007/s12035-013-8571-y

AbbkineGSK3β Mouse Monoclonal antibody (1A6) helps basic research! Basic information about the product:

Product name	#CAT	Reactivity	Applications	Dilution ratio	Observed Band(KD)
GSK3β Mouse Monoclonal antibody (1A6)	ABM0076	human/mouse/rat	WB/IHC-P	WB (1:1000-1:2000) IHC-P (1:100-1:200)	46 kD

GSK3β Mouse Monoclonal antibody (1A6)
verification results:

Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using GSK3β Mouse mAb diluted at 1:200.

Immunohistochemical analysis of paraffin-embedded Human Stomach Carcinoma Tissue using GSK3β Mouse mAb diluted at 1:200.

Western blot analysis of 1) Hela Cell Lysate, 2) 3T3 Cell Lysate, 3) Rat Brain Tissue Lysate using GSK3β Mouse mAb diluted at 1:1000.

Selected primary antibody| Cleaved Caspase-3 antibody recommendation

Review of Related Articles:

Research
Hotspots of Cell Apoptosis: Bcl-2 Monoclonal Antibody

Abbkine
P53 antibody helps basic cancer research!

Marker
of myofibroblast antibody-α-SMA/ACTA2 Monoclonal Antibody

Selected
primary antibody|TNF-α antibody recommendation

Cleaved Caspase-3 is an Activated Form of
Caspase-3. Caspases are a family of cysteine proteases that are key mediators
of programmed cell death or apoptosis. The precursor form of all caspases is
composed of a prodomain, and large and small catalytic subunits. The active
forms of caspases are generated by several stimuli including ligand-receptor
interactions, growth factor deprivation and inhibitors of cellular functions.

All known caspases require cleavage
adjacent to aspartates to liberate one large and one small subunit, which
associate into a2b2 tetramer to form the active enzyme. Gene for Caspase-3 also
known as Yama, CPP32, and apopain codes for a 32-kDa protein. The main
substrate of Caspase-3 is poly (ADP- ribose) polymerase PARP (ADP-ribose) which
is related to DNA repair and gene integrity monitoring. When apoptosis is
initiated, Caspase-3 is activated by cleavage of Asp-28/Ser-29 (between
N-terminal prodomains) and Asp-175/Ser-176 (between large subunit and small
subunit), resulting in a large subunit of 17kDa and a small subunit of 12kDa.
P17 and p12 dimerize to form cleaved caspase-3 in activated form.

The picture is from https://www.nature.com/articles/cdd2009180.

AbbkineCleaved-Caspase-3
p17 (D175) Polyclonal Antibody helps basic cancer research! Basic information
about the product:

Product name	#CAT	Reactivity	Applications	Dilution ratio	Observed Band(KD)
Cleaved-Caspase-3 p17 (D175) Polyclonal Antibody	ABP0021	human/mouse/rat	WB/IF/IHC-P/ELISA	WB (1:500-1:2000) IF (1:50-1:300) IHC-P (1:50-1:300) ELISA（1:20000）	19/17 kD

Abbkine Cleaved-Caspase-3 p17 (D175)
Polyclonal Antibody verification results:

Western Blot analysis using Abbkine Cleaved-Caspase-3 p17 (D175) Polyclonal Antibody.

Immunohistochemical analysis of paraffin-embedded mouse colon tissue (1:200) using Abbkine Cleaved-Caspase-3 p17 (D175) Polyclonal Antibody. (1:200).

Immunohistochemical analysis of paraffin-embedded mouse colon tissue (1:200) using Abbkine Cleaved-Caspase-3 p17 (D175) Polyclonal Antibody. (1:200).

Selected primary antibody|TNF-α antibody recommendation

Review of Related Articles:

Research
Hotspots of Cell Apoptosis: Bcl-2 Monoclonal Antibody

Abbkine
P53 antibody helps basic cancer research!

Marker of myofibroblast antibody-α-SMA/ACTA2 Monoclonal Antibody

Tumor necrosis factor (TNF, cachexin, or
cachectin; once named as tumor necrosis factor alpha or TNFα) is a cell
signaling protein (cytokine) involved in systemic inflammation and is one of
the cytokines that make up the acute phase reaction. It is produced chiefly by
activated macrophages, although it can be produced by many other cell types
such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and
neurons. TNF is a member of the TNF superfamily, consisting of various
transmembrane proteins with a homologous TNF domain.

The primary role of TNF is in the regulation of immune cells. TNF, being an endogenous pyrogen, is able to induce fever, apoptotic cell death, cachexia, inflammation and to inhibit tumorigenesis and viral replication and respond to sepsis via IL1- & IL6-producing cells. Dysregulation of TNF production has been implicated in a variety of human diseases including Alzheimer's disease, cancer, major depression, psoriasis and inflammatory bowel disease (IBD). Though controversial, studies of depression and IBD are currently being linked to increased levels of TNF. Recombinant TNF is used as an immunostimulant under the INN tasonermin. TNF can be produced ectopically in the setting of malignancy and parallels parathyroid hormone both in causing secondary hypercalcemia and in the cancers with which excessive production is associated.

Fig: Anti-TNF-α therapies: the next generation (https://www.nature.com/articles/nrd1175)

Abbkine TNF-α
Polyclonal Antibody helps basic cancer research! Basic information about
the product:

Product name	#CAT	Reactivity	Applications	Dilution ratio	Observed Band(KD)
TNF-α Polyclonal Antibody	ABP0127	Human/Mouse/Rat	WB/IF/IHC-P/ELISA	WB (1:500-1:2000) IF (1:200-1:1000) IHC-P (1:100-1:300) ELISA（1/10000）	16KD

Abbkine TNF-α Polyclonal Antibody verification results:

Western Blot analysis using Abbkine TNF-α Polyclonal Antibody.

Immunofluorescence analysis of human stomach tissue using Abbkine TNF-α Polyclonal Antibody (1:200).

Immunohistochemical analysis of paraffin-embedded Human stomach (1:100).

Marker of myofibroblast antibody-α-SMA/ACTA2 Monoclonal Antibody

ACTA2 (actin alpha 2) is an actin protein
with several aliases including alpha-actin, alpha-actin-2, aortic smooth muscle
or alpha smooth muscle actin (α-SMA, SMactin, alpha-SM-actin, ASMA). Actins are
a family of globular multi-functional proteins that form microfilaments. ACTA2
is one of 6 different actin isoforms and is involved in the contractile
apparatus of smooth muscle. ACTA2 (as with all the actins) is extremely highly
conserved and found in nearly all mammals.

In humans, ACTA2 is encoded by the ACTA2
gene located on 10q22-q24. Mutations in this gene cause a variety of vascular
diseases, such as thoracic aortic disease, coronary artery disease, stroke,
Moyamoya disease, and multisystemic smooth muscle dysfunction syndrome.

ACTA2 (commonly referred to as alpha-smooth muscle actin or α-SMA) is often used as a marker of myofibroblast formation.

Figure: Diverse origins of the
myofibroblast—implications for kidney fibrosis (From Nature Reviews)

Abbkine α-SMA Monoclonal Antibody Helps Basic Cancer Research! Basic information about the product:

Product name	#CAT	Reactivity	Applications	Dilution ratio	Observed Band(KD)
α-SMA Monoclonal Antibody	ABM0052	Human/Mouse/ Rat	WB/IF/IHC-P	WB (1:10000-1:100000) IF (1:100-1:200) IHC-P (1:1000-1:2000)	42KD

Abbkine α-SMA Monoclonal Antibody verification results:

	Western blot analysis of 1) Hela, 2) 3T3, 3) rat brain using α-SMA Monoclonal Antibody.
	Immunofluorescence analysis of human liver tissue.
	Immunohistochemical analysis of paraffin-embedded mouse liver tissue

Abbkine winter promotion: looking for you who need live-cell probe

Promotion time: From now to 15^th January, 2020.

Promotion product
list: TraKine™ Pro product
line (Tubulin, Lysosome, Mitochondrion, Nuclei live-cell probe ).

Details of activities: 20% off for TraKine™ Pro product line.

Details for promotion product:

Product name	#Cat	Target	Ex/Em (nm)	Size	Promotion price ($)
TraKine™ Pro Live-cell Tubulin Staining kit (Green Fluorescence with Super Resolution)	KTC4100	Tubulin	500/520	50/250T	144/496
TraKine™ Pro Live-cell Lysosome Staining kit (Deep Red Fluorescence with Super Resolution)	KTC4210	Lysosome	650/665	50/250T	128/472
TraKine™ Pro Live-cell Lysosome Staining kit (Orange Fluorescence with Super Resolution)	KTC4220	Lysosome	565/590	50/250T	128/4721
TraKine™ Pro Live-cell Mitochondrion Staining kit (Deep Red Fluorescence with Super Resolution)	KTC4300	Mitochondrion	647/661	50/250T	176/576
TraKine™ Pro Live-cell Nuclei Staining kit (Deep Red Fluorescence with Super Resolution)	KTC4510	Nuclei	650/665	50/250T	144/496

TraKine™ Pro features & benefits:

• Proprietary probe contains fluorescent dye and a unit which
  electively recognize organelles, with high specificity, low background,
  excellent photostability and good cell permeability.
• Optimized staining protocol for labeling subcellular structures in
  mammalian living and fixed cells.
• Fuorescence can last for several hours in cells, stable and
  persistent, especially ideal for monitoring dynamics.
• Super resolution, especially suitable for Confocal and long-term
  super-resolution imaging (such as SIM, STED, TIRF, STORM and PALM).
• Safe, very low or no cytotoxicity to cells.