Ub Polyclonal Antibody Review

Ubiquitin is a highly conserved 76kDa protein which is ligated to many proteins in both monomeric and polymeric forms. It is involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. Ubiquitin polymers (polyubiquitin) can be found in several varieties depending on which of its 7 lysine residues is the site of ligation. The two most common polymer chains are linked via an isopeptide bond between glycine-76 and either lysine-48 (K-48) or lysine-63 (K-63).

Ub Polyclonal Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This antibody has been tested with WB，IHC-p and ELISA. And Abbkine suggested starting dilutions are as follows: WB: 1:500-1:2000, IHC-p: 1:100-1:300, ELISA: 1:10000. Not yet tested in other applications.

Ubiquitin is a highly conserved nuclear and cytoplasmic protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome. It applies to WB，IHC-p and ELISA experiments. It's convenient to choose this product.

Anti-Plant Actin Mouse Monoclonal Antibody (3T3), another scientific breakthrough from Abbkine Scientific Company Limited

Abbkine Scientific Company based in China recently announced the release of its new product, the Anti-Plant Actin Mouse Monoclonal Antibody (3T3), an antibody released for research use, with reactivity in plants.

One of the highly conserved proteins that make up an essential part of cell cytoskeleton is Actin. The substance also plays a significant role in the streaming of cytoplasm, determination of cell shape, division of cell, loading control, extension growth, and organelle movement. ACT2 and ACT8 represent one of the complex actin gene family encoding six protein subclasses. The flanking sequences of these proteins are however different from their peers, with introns and silent nucleotide positions in codons. The ACT8 protein is expressed in leaves, roots, stems flowers, siliques, and pollen.

Anti-Plant Actin Mouse Monoclonal Antibody (3T3) is monoclonal, with a WB application, and a Mouse IgG1 isotype. It is best applied for optimal working conditions after the experimental determination by the investigator. It is however suggested that starting dilutions are applied WB 1:2000-5000.

The antibody was specially purified from mouse ascites with the affinity purification method by affinity-chromatography, with the use of specific immunogen. The liquid solution scientific research product comes in a liquid solution and has a storage buffer of Liquid in PBS, pH 7.4, containing 0.02% sodium azide as preservative and 50% Glycerol. The antibody can be stored for as long as a year after the date of shipment. It is however advised that the product remains in a stable temperature of -20°C from the date it was shipped through the storage period. Users should centrifuge the original vial after thawing and prior to removing the cap, for the best recovery of the product.

Abbkine Scientific Company Limited has expressly warned against using the plant actin for use in human, clinical diagnosis, violation of any patent or as a license.

About Abbkine Scientific Co., Ltd

Abbkine Scientific Co., Ltd was founded in California in 2012 by a group of scientists and marketing experts in the life science field. The company since it was founded has provided innovative, high quality and cost-effective life science research and diagnosis products which have led to its steady and significant growth.

The company aims to continue to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, and drug discovery, with the combination of cutting edge technology from United States with China's manufacturing engineering and cost advantages.

Abbkine is determined to advance human and animal health by harnessing the best of human resources in combination with state-of-the-art technology. Since its founding, the company has been able to provide the highest quality products at affordable prices, some of which are listed below.

• Full portfolio of epitope antibodies and internal control antibodies with custom conjugates;


• Full portfolio of phospho-specific and featured methylation-specific antibodies with small size;


• Top sale secondary antibodies with IFKine series for IF and IPkine for IP-WB analysis;


• Full portfolio of protein purification tools with high quality and competitive price;


• Top sale recombinant cytokines and growth factors;


• More Cell function analysis kits, ELISA kits and cell tracking reagents are coming soon

Integrin β3 Polyclonal Antibody Review

ITGB3 integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIB/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIB/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIB/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha- IIB/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface.

Integrin β3 Polyclonal Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This antibody has been tested with WB and ELISA. And Abbkine suggested starting dilutions are as follows: WB: 1:500-1:2000, ELISA: 1:10000. Not yet tested in other applications.

We always have faith in the quality of Abbkine's products. The pictures are beautiful! It is worth recommending.

 

Abbkine wishes Happy New Year to all the friends !

Happy New Year, and thank you to our partners for helping make 2016 another groundbreaking year for Abbkine. We wish you all the best for the year 2017 !

Chinese New Year is the longest and most important celebration in the Chinese calendar. On the 28^th January, it will be the year of the Rooster and many Chinese people will be celebrating at home. For Abbkine, new year means starting afresh.

The year 2016 is a special year for Abbkine. With growing demands from Asia Pacific, Abbkine moves its headquarters to China. We expand our portfolio and establish more perfect distributor system.

We have created substantial momentum for 2016. In addition to us focusing on our key growth areas of antibodies, you have seen recombinant proteins, assay kits, biochemicals and purification tools , with many of you helping to make that happen. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we'll continue to provide innovative, high quality other research tools to accelerate life science fundamental research, drug discovery, etc.

During 2016, we published a lot of posts on the Abbkine website, facebook, twitter and other social network. With our professional E-commerce advantages and more than $ 4000 / month of the continued growth of the marketing budget, we gives our partners further assistance in getting new products to market. We'll expand our global market further in 2017 and let Abbkine services more scientific researchers.

Thank you 2016, welcome 2017. We look forward to the new year and everything it may bring. We hope you join us and develop together !

VE-Cadherin Polyclonal Antibody Review

VE-Cadherin is an alias for the gene, Cadherin 5, type 2 (vascular endothelium), in humans. The encoded protein has an amino acid length of 784 and a mass of 87.5 kDa. Other isoforms may exist. This gene shares homology with other species. This gene encodes a classical cadherin of the cadherin superfamily. The encoded preproprotein is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion molecule is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classical cadherin by imparting to cells the ability to adhere in a homophilic manner, this protein plays a role in endothelial adherens junction assembly and maintenance.

VE-Cadherin Polyclonal Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This antibody has been tested with WB and ELISA. And Abbkine suggested starting dilutions are as follows: WB: 1:500-1:2000, ELISA: 1:10000. Not yet tested in other applications.

VE-Cadherin is located in a gene cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. I chose this product. And I am quite satisfied.

CYCS Polyclonal Antibody Released

CYCS gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in CYCS gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.

Abbkine CYCS  Polyclonal Antibody was affinity-purified from rabbit serum by affinity-chromatography using synthetic peptide from human protein at AA range: 1268-1341. The verified reactivities are Human, Mouse and Rat. Test confirmed the antibody can be applied in  ELISA, IHC-p, WB. Abbkine suggested the starting dilutions are as follows: WB: 1:500-1:2000, IHC-p: 1:50-1:300, ELISA 1:5000-1:20000.

Supplied as liquid solution at 1 mg/ml, the antibody is stable for one year at -20°C from the date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

p16 Polyclonal Antibody Review

Cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4). Cyclin-dependent kinase inhibitor 2A interacts strongly with cdk4 and cdk6. It belongs to the cdkn2 family of cyclin-dependent kinase inhibitors, and plays role in cyclins and cell cycle regulation.CDKN2A acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This interaction inhibits CDKs binding to cyclins D and phosphorylation of the retinoblastoma protein. Defects in CDKN2A are the cause of numerous cancers such as melanoma and astrocytoma.

p16 Polyclonal Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This antibody has been tested with WB and ELISA. And Abbkine suggested starting dilutions are as follows: WB: 1:500-1:2000, ELISA: 1:10000. Not yet tested in other applications.

CDKN2A is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene. It applies to WB and ELISA experiments. It's convenient to choose this product.

Abbkine Scientific Announces Release of a New Antibody - Anti-GAPDH Mouse Monoclonal Antibody (2B5)

The new discovery made by scientific research company, Abbkine Scientific Company Limited is another milestone achieved in the science world, with the announcement of the Anti-GAPDH Mouse Monoclonal Antibody (2B5).

The monoclonal antibody otherwise known as GAPDH comes in a liquid solution. Glyceraldehyde 3-phosphate dehydrogenase, is a ~37kDa enzyme, synonymous with crystallizing the sixth step of glycosis and subsequently helping with the breakdown of energy and carbon molecules. The Peptidyl-cysteine S-nitrosylase GAPDH in addition to these function, also helps in a number of non-metabloc processes that include transcription activation, initiation of apoptosis ER to Golgi vesicle shuttling, and fast axonal, or axoplasmic transport.

The release of the product is therefore a giant stride in the world of science. The applications of the antibody are IHC and WB, with a synthetic peptide immunogen. the product’s reactivity is in Chicken, Dog, Human, Insect, Monkey, Mouse, Pig, Rabbit, Rat, Sheep, and Yeast, hosted in mouse, and therefore having a Mouse IgG1 isotype. Its Gene ID is 2597, with a P04406 accession.

For optimal results, it is advised that the investigator experimentally determines the best working dilutions, with suggested starting dilutions stated as WB 1:5000, IHC-p 1:200. The product was affinity-purified from mouse ascites, with the affinity-chromatography method, using specific immunogen.

The product is best stored at -20°C, with guaranteed one year stability from the date of shipment. It is advised that the original vial is centrifuged after thawing and before removing the cap, for maximum recovery of the product. Shipping should be done gel-packed with blue ice.

The product is made for specifically for research use and should not be administered for use in human or clinical diagnosis.

About Abbkine Scientific Co., Ltd

Abbkine Scientific Co., Ltd was founded in California in 2012 by a group of scientists and marketing experts in the life science field. The company since it was founded has provided innovative, high quality and cost-effective life science research and diagnosis products which have led to its steady and significant growth.

The company aims to continue to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, and drug discovery, with the combination of cutting edge technology from United States with China's manufacturing engineering and cost advantages.

Abbkine is determined to advance human and animal health by harnessing the best of human resources in combination with state-of-the-art technology. Since its founding, the company has been able to provide the highest quality products at affordable prices, some of which are listed below.

• Full portfolio of epitope antibodies and internal control antibodies with custom conjugates;


• Full portfolio of phospho-specific and featured methylation-specific antibodies with small size;


• Top sale secondary antibodies with IFKine series for IF and IPkine for IP-WB analysis;


• Full portfolio of protein purification tools with high quality and competitive price;


• Top sale recombinant cytokines and growth factors;


• More Cell function analysis kits, ELISA kits and cell tracking reagents are coming soon

100% Quality guarantee

Customer satisfaction at Abbkine is our priority. Every product you purchase from Abbkine is covered by a 6-month satisfaction guarantee that it will work in the application(s) and species described in the user guide or datasheet. Here is the sequence of processes we take to help ensure your satisfaction:

• Expert technical support to troubleshoot the issue


• Discount or credit towards a future purchase of the same or similar product


• Replacement of product components or the product in its entirety


• Product credit, store credit, or refund of the original purchase price

Terms & Conditions

• Guarantee is valid for 6 months from date of delivery.


• Guarantee is valid for products purchased direct from Abbkine or through an official, authorized distributor. Additional conditions may apply from distributor.


• Instruments and plastics are subject to its own guarantee and warranty policies.


• "Predicted to react" and "broad range" species are based on sequence homology and have not been extensively tested, and are not applicable under this guarantee.


• Account must be in good standing for guarantee offer to apply. Non-payment of any outstanding invoice(s) voids this guarantee.


• Experimental data and raw data values are required in order to receive replacement, store credit, or refund under this guarantee.


• User modifications to the protocol of products including those relating to usage of techniques, input material, and equipment voids this guarantee.

Caspase-1 Polyclonal Antibody Review

Caspase-1 (also known as ICE) is a prototypical member of the caspase-family of cysteine proteases. Caspase-1 exists in cells as an inactive 45 kDa proenzyme. The pro-enzyme is matured by proteolysis to yield large (20 kDa) and small (10 kDa) subunits. The active caspase-1 is a heterotetramer consisting of two large and two small subunits. To date the regulatory mechanism of caspase-1 activation and the role of caspase-1 in apoptosis are poorly understood. In THP-1 cells, a large proportion of the caspase-1 is present in the inactive proenzyme form.

Caspase-1 Polyclonal Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This antibody has been tested with WB and ELISA. And Abbkine suggested starting dilutions are as follows: WB: 1:500-2000, ELISA 1：10000-20000.

Caspase-1 is a mediator of the inflammatory response by cleaving the inactive pro-IL1 beta at Asp116/Ala 117 to generate the active 17k mature form of IL-1 beta. We find the quality suitable for our experiments. I am very satisfied with Abbkine's service.

TERT Polyclonal Antibody Released

Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. hTERT (TERT) is the rate-limiting catalytic subunit of telomerase. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks.

Abbkine TERT Polyclonal Antibody was affinity-purified from rabbit serum by affinity-chromatography using synthetic peptide from human protein at AA range: 1268-1341. The verified reactivity and applications respectively are human and ELISA, IHC-p, WB. Abbkine suggested the starting dilutions are as follows: WB: 1:500-1:2000, IHC-p: 1:50-1:300, ELISA 1:5000-1:20000.

Supplied as liquid solution at 1 mg/ml, the antibody is stable for one year at -20°C from the date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

PTEN Polyclonal Antibody Review

PTEN (phosphatase and tensin homologue deleted on chromosome ten), also referred to as MMAC (mutated in multiple advanced cancers) phosphatase, is a tumor suppressor implicated in a wide variety of human cancers. PTEN encodes a 403 amino acid polypeptide originally described as a dual-specificity protein phosphatase. The main substrates of PTEN are inositol phospholipids generated by the activation of the phosphoinositide 3-kinase (PI3K). PTEN is a major negative regulator of the PI3K/Akt signaling pathway. PTEN possesses a carboxy-terminal, noncatalytic regulatory domain with three phosphorylation sites (Ser380, Thr382, and Thr383) that regulate PTEN stability and may affect its biological activity. PTEN regulates p53 protein levels and activity and is involved in G protein-coupled signaling during chemotaxis.

PTEN Polyclonal Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This antibody has been tested with WB and ELISA. And Abbkine suggested starting dilutions are as follows: WB: 1:500-2000, ELISA 1：10000-20000.

PTEN (Phosphatase And Tensin Homolog) is a Protein Coding gene. Diseases associated with PTEN include Bannayan-Riley-Ruvalcaba Syndrome and Macrocephaly/Autism Syndrome. This product is what I need. I've tried PTEN Polyclonal Antibody with excellent results！

Browse Abbkine featured 8000 antibodies on Antibody-Adviser

Antibody-Adviser is a association developing a unique platform to enable fast and efficient information sharing on any antibody used for research. Antibody-Adviser aims to make scientist's life easy and improve the laboratory experience! Recently, Abbkine Scientific post their featured 8000 antibodies on Antibody-Adviser website aiming to help make research possible by supplying scientists with the basic research tools necessary for advancing human and animal health.
Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Abbkine aims to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate drug discovery in disease-related areas like cancer, diabetes, obesity, Alzheimer’s disease, etc.

Browse Abbkine featured 8000 antibodies on Antibody-Adviser

Antibody-Adviser is a association developing a unique platform to enable fast and efficient information sharing on any antibody used for research. Antibody-Adviser aims to make scientist's life easy and improve the laboratory experience! Recently, Abbkine Scientific post their featured 8000 antibodies on Antibody-Adviser website aiming to help make research possible by supplying scientists with the basic research tools necessary for advancing human and animal health.
Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Abbkine aims to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate drug discovery in disease-related areas like cancer, diabetes, obesity, Alzheimer’s disease, etc.

Browse Abbkine featured 8000 antibodies on Antibody-Adviser

Antibody-Adviser is a association developing a unique platform to enable fast and efficient information sharing on any antibody used for research. Antibody-Adviser aims to make scientist's life easy and improve the laboratory experience! Recently, Abbkine Scientific post their featured 8000 antibodies on Antibody-Adviser website aiming to help make research possible by supplying scientists with the basic research tools necessary for advancing human and animal health.
Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Abbkine aims to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate drug discovery in disease-related areas like cancer, diabetes, obesity, Alzheimer’s disease, etc.

Browse Abbkine featured 8000 antibodies on Antibody-Adviser

Antibody-Adviser is a association developing a unique platform to enable fast and efficient information sharing on any antibody used for research. Antibody-Adviser aims to make scientist's life easy and improve the laboratory experience! Recently, Abbkine Scientific post their featured 8000 antibodies on Antibody-Adviser website aiming to help make research possible by supplying scientists with the basic research tools necessary for advancing human and animal health.
Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Abbkine aims to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate drug discovery in disease-related areas like cancer, diabetes, obesity, Alzheimer’s disease, etc.

Browse Abbkine featured 8000 antibodies on Antibody-Adviser

Antibody-Adviser is a association developing a unique platform to enable fast and efficient information sharing on any antibody used for research. Antibody-Adviser aims to make scientist's life easy and improve the laboratory experience! Recently, Abbkine Scientific post their featured 8000 antibodies on Antibody-Adviser website aiming to help make research possible by supplying scientists with the basic research tools necessary for advancing human and animal health.
Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Abbkine aims to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate drug discovery in disease-related areas like cancer, diabetes, obesity, Alzheimer’s disease, etc.

Browse Abbkine featured 8000 antibodies on Antibody-Adviser

Antibody-Adviser is a association developing a unique platform to enable fast and efficient information sharing on any antibody used for research. Antibody-Adviser aims to make scientist's life easy and improve the laboratory experience! Recently, Abbkine Scientific post their featured 8000 antibodies on Antibody-Adviser website aiming to help make research possible by supplying scientists with the basic research tools necessary for advancing human and animal health.
Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Abbkine aims to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate drug discovery in disease-related areas like cancer, diabetes, obesity, Alzheimer’s disease, etc.

Browse Abbkine featured 8000 antibodies on Antibody-Adviser

Antibody-Adviser is a association developing a unique platform to enable fast and efficient information sharing on any antibody used for research. Antibody-Adviser aims to make scientist's life easy and improve the laboratory experience! Recently, Abbkine Scientific post their featured 8000 antibodies on Antibody-Adviser website aiming to help make research possible by supplying scientists with the basic research tools necessary for advancing human and animal health.
Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Abbkine aims to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate drug discovery in disease-related areas like cancer, diabetes, obesity, Alzheimer’s disease, etc.

Browse Abbkine featured 8000 antibodies on Antibody-Adviser

Antibody-Adviser is a association developing a unique platform to enable fast and efficient information sharing on any antibody used for research. Antibody-Adviser aims to make scientist's life easy and improve the laboratory experience! Recently, Abbkine Scientific post their featured 8000 antibodies on Antibody-Adviser website aiming to help make research possible by supplying scientists with the basic research tools necessary for advancing human and animal health.
Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Abbkine aims to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate drug discovery in disease-related areas like cancer, diabetes, obesity, Alzheimer’s disease, etc.

Browse Abbkine featured 8000 antibodies on Antibody-Adviser

Antibody-Adviser is a association developing a unique platform to enable fast and efficient information sharing on any antibody used for research. Antibody-Adviser aims to make scientist's life easy and improve the laboratory experience! Recently, Abbkine Scientific post their featured 8000 antibodies on Antibody-Adviser website aiming to help make research possible by supplying scientists with the basic research tools necessary for advancing human and animal health.
Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Abbkine aims to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate drug discovery in disease-related areas like cancer, diabetes, obesity, Alzheimer’s disease, etc.

Browse Abbkine featured 8000 antibodies on Antibody-Adviser

Antibody-Adviser is a association developing a unique platform to enable fast and efficient information sharing on any antibody used for research. Antibody-Adviser aims to make scientist's life easy and improve the laboratory experience! Recently, Abbkine Scientific post their featured 8000 antibodies on Antibody-Adviser website aiming to help make research possible by supplying scientists with the basic research tools necessary for advancing human and animal health.
Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Abbkine aims to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate drug discovery in disease-related areas like cancer, diabetes, obesity, Alzheimer’s disease, etc.

Browse Abbkine featured 8000 antibodies on Antibody-Adviser

Antibody-Adviser is a association developing a unique platform to enable fast and efficient information sharing on any antibody used for research. Antibody-Adviser aims to make scientist's life easy and improve the laboratory experience! Recently, Abbkine Scientific post their featured 8000 antibodies on Antibody-Adviser website aiming to help make research possible by supplying scientists with the basic research tools necessary for advancing human and animal health.
Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Abbkine aims to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate drug discovery in disease-related areas like cancer, diabetes, obesity, Alzheimer’s disease, etc.

IFKine™ Red Donkey Anti-Rabbit IgG Released

IFKine™ Red conjugated antibodies absorb light maximally around 591 nm and fluoresce with a peak around 615 nm. They are brighter, more photostable, and more hydrophilic than other red-fluorescing conjugates. They are the best choice for immunofluorescence detection in the deep-red region of the visible spectrum.

IFKine™ Red Donkey Anti-Rabbit IgG reacts with whole molecule rabbit IgG. It also reacts with light chains of all other rabbit immunoglobulins. It's cross-reaction with human, mouse, rat, goat, sheep and guinea pig serum proteins has been specialized minimized to reduce non-specific hybrization. It has no reactivity on non-immunoglobulin serum proteins. Abbkine suggested the starting dilutions for most applications are 1:50-1:1,000.

Abbkine IFKine™ is series of unique fluorence staining secondary antibodies with improved brightness, photostability and less nonspecific hybridization and background. The latest generation of IFKine fluorescent dyes ensure the best fluorescent performance, while it’s donkey host and other species of serum/IgG absorbed make IFKine™ secondary antibodies the ideal for use in fluorence staining, especially in fluorence multiple labeling.

IFKine™ Red Donkey Anti-Mouse IgG antibody is also supplied by Abbkine.

NGF Polyclonal Antibody Review

Nerve growth factor (NGF) is a small secreted protein that is important for the growth, maintenance, and survival of certain target neurons (nerve cells). Producing cells release NGF that bind and activate TrkA high affinity receptors to mediate NGF-driven signaling. NGF also binds to a low affinity p75 (NTR) receptors, which belong to the death receptor family. Although NGF has been classically described as favoring neuron survival and differentiation, nerve growth factor can promote apoptosis in cells that contain p75 (NTR) and lack TrkA. NGF can induce neuron death in a variety of neurodegenerative conditions, including Alzheimer disease. Besides its neurotrophic actions, NGF has an effect on non-neuronal cells and may help mediate inflammation, angiogenesis, and stimulate breast cancer cell growth.

NGF Polyclonal Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This antibody has been tested with WB and ELISA. And Abbkine suggested starting dilutions are as follows: WB: 1:500-1:2000, ELISA: 1:10000. Not yet tested in other applications.

NGF signaling is looking increasingly promising as potential drug targets for diseases. It is responsible to say that NGF Polyclonal Antibody has a reliable quality. I am very happy that I chose this product.

Abbkine Scientific announces the release of Anti-beta-Actin Mouse Monoclonal Antibody (1C7)

Abbkine Scientific Company Limited recently announced the release of a new product called Anti-β-Actin Mouse Monoclonal Antibody (1C7), a synthetic peptide hosted by the mouse. The liquid solution, released singularly for research use and not for human or clinical use, is another giant stride from one of the world’s leading research and scientific company.

ACTB is one of the major components of the cytoskeleton with a minimum of six isoforms known in mammals. Actins are generally important in the area of cell structure, motility, and integrity, and β-actin constitutes a major part of the contractile apparatus used as a loading control.

The applications of the product include IHC and WB, with optimal working dilutions determined by the investigator experimentally. It is however suggested that the dilutions are started as WB 1:5000, IHC-p 1:200 to get the best result possible.

Otherwise known as ACTB; Actin; cytoplasmic 1; Beta-actin, with a synthetic peptide immunogen, the anti-body’s reactivity has been seen on chicken, rabbit, rat, monkey, insects, mouse, and even human. The product was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

The product can be stored for one year at a stable condition of -20°C from the shipment date. To ensure the product is maximally recovered, it is advised that the original vial is centrifuged after thawing and before the cap is removed.

About Abbkine Scientific Co., Ltd

Abbkine Scientific Co., Ltd was founded in California in 2012 by a group of scientists and marketing experts in the life science field. The company since it was founded has provided innovative, high quality and cost-effective life science research and diagnosis products which have led to its steady and significant growth.

The company aims to continue to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, and drug discovery, with the combination of cutting edge technology from United States with China's manufacturing engineering and cost advantages.

Abbkine is determined to advance human and animal health by harnessing the best of human resources in combination with state-of-the-art technology. Since its founding, the company has been able to provide the highest quality products at affordable prices, some of which are listed below.

• Full portfolio of epitope antibodies and internal control antibodies with custom conjugates;


• Full portfolio of phospho-specific and featured methylation-specific antibodies with small size;


• Top sale secondary antibodies with IFKine series for IF and IPkine for IP-WB analysis;


• Full portfolio of protein purification tools with high quality and competitive price;


• Top sale recombinant cytokines and growth factors;


• More Cell function analysis kits, ELISA kits and cell tracking reagents are coming soon

Bcl-2 Polyclonal Antibody Review

BCL2 (B-cell leukemia/lymphoma 2, N-Histidine-tagged), also known as Bcl-2, belongs to the Bcl-2 family. Bcl-2 family proteins regulate and contribute to programmed cell death or apoptosis. It is a large protein family and all members contain at least one of four BH (bcl-2 homology) domains. Certain members such as Bcl-2, Bcl-xl and Mcl1 are anti-apoptotic, whilst others are pro-apoptotic. Most Bcl-2 family members contain a C-terminal transmembrane domain that functions to target these proteins to the outer mitochondrial and other intracellular membranes. It is expressed in a variety of tissues. BCL2 blocks the apoptotic death of some cells such as lymphocytes. It also regulates cell death by controlling the mitochondrial membrane permeability and inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.

Bcl-2 Polyclonal Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This antibody has been tested with WB and ELISA. And Abbkine suggested starting dilutions are as follows: WB: 1:500-2000, ELISA 1：10000-20000.

BCL2(BCL2, apoptosis regulator)encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. The quality of Bcl-2 Polyclonal Antibody is found satisfactory. Thanks a lot for the help of your company!

E2F-1 Polyclonal Antibody Review

The protein encoded by E2F1（E2F transcription factor 1） is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. E2F's are DNA binding proteins, which associate with negative regulators, such as the retinoblastoma p107 protein, resulting in an altered rate of gene transcription. E2F1 is proposed to be involved in several cellular processes that range from tumor suppressor, cell progression and oncogenesis. E2F1 overexpression can also drive cells into apoptosis.

E2F-1 Polyclonal Antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. This antibody has been tested with WB and ELISA. And Abbkine suggested starting dilutions are as follows: WB: 1:500-10000, ELISA: 1:10000.

Abbkine means not only a good quality but also a service and a reliable market reputation. We always have faith in the quality of Abbkine's products. We find the quality suitable for our experiments.

IFKine™ Orange Donkey Anti-Mouse IgG Released

IFKine™ Orange conjugated antibodies absorb light maximally at 555 nm and fluoresce with a peak around 570 nm. They are brighter than TRITC, Cy3. IFKine™ Orange conjugates are recommended for for all immunofluorescence procedures requiring a orange -fluorescing dye.

IFKine™ Orange Donkey Anti-Mouse IgG antibody reacts with whole molecule mouse IgG. It also reacts with light chains of all other mouse immunoglobulins. It's cross-reaction with human, rabbit, goat, sheep and guinea pig serum proteins has been specialized minimized to reduce non-specific hybrization. It has no reactivity on non-immunoglobulin serum proteins. Abbkine suggested the starting dilutions for most applications are 1:50-1:1,000 .

Abbkine’s IFKine® is series of unique fluorence staining secondary antibodies with improved brightness, photostability and less nonspecific hybridization and background. The latest generation of IFKine fluorescent dyes ensure the best fluorescent performance, while it’s donkey host and other species of serum/IgG absorbed make IFKine® secondary antibodies the ideal for use in fluorence staining, especially in fluorence multiple labeling.

TERT Polyclonal Antibody Review

TERT is a symbol for the gene, Telomerase reverse transcriptase, in humans. Other names include: Telomerase catalytic subunit; Telomerase-associated protein 2; HEST2; Telomerase reverse transcriptase ; CMM9; DKCA2; DKCB4; EST2; PFBMFT1; TRT; hTRT. The encoded protein is an enzyme with an amino acid length of 1132 and a mass of 127.0 kDa. TERT is a member of the Reverse transcriptase family, Telomerase subfamily.

TERT Polyclonal Antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. This antibody has been tested with WB, IHC-p and ELISA. And Abbkine suggested starting dilutions are as follows: WB: 1:500-1:2000,IHC-p: 1:50-1:300,ELISA 1:5000-1:20000.

This gene shares homology with other species, including: Mouse. We make experiments with this TERT Antibody. The results of the experiment is satisfactory.

IFKine™ Green Donkey Anti-Goat IgG Released

IFKine™ Green conjugated antibodies absorb light maximally at 493 nm and fluoresce with a peak around 518 nm. They are brighter than FITC, Cy2, and DyLight 488. IFKine™ Green conjugates are recommended for for all immunofluorescence procedures requiring a green-fluorescing dye.

IFKine™ Green Donkey Anti-Goat IgG antibody reacts with whole molecule goat IgG. It also reacts with light chains of all other goat immunoglobulins. It's cross-reaction with human, mouse, rat, rabbit and guinea pig serum proteins has been specialized minimized to reduce non-specific hybrization. It has no reactivity on non-immunoglobulin serum proteins. Optimal working dilutions should be determined experimentally by the investigator. Abbkine suggested the starting dilution is  1:50-1:1,000 for most applications.

Abbkine’s IFKine® is series of unique fluorence staining secondary antibodies with improved brightness, photostability and less nonspecific hybridization and background. The latest generation of IFKine fluorescent dyes ensure the best fluorescent performance, while it’s donkey host and other species of serum/IgG absorbed make IFKine® secondary antibodies the ideal for use in fluorence staining, especially in fluorence multiple labeling.

Need IFKine™ Green Donkey Anti-Mouse IgG or IFKine™ Green Donkey Anti-Rabbit IgG, please visit www.abbkine.com.

CiteAb cited full portfolio of Abbkine featured antibodies and publications

CiteAb is the largest citation-ranked antibody search engine and database. It provides an impartial way to find and compare antibodies that work. CiteAb use our database of antibody publications to produce unique antibody market data, income from this data allows us to remain free to list and impartial. Check Abbkine featured 8000 antibodies from CiteAb website.

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. We aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate drug discovery in disease-related areas like cancer, diabetes, obesity, Alzheimer's disease, etc. Our mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. We would achieve these goals through a well-defined core strategy and a company culture that fosters integrity, openness, and collaboration, and rewards customer focus, innovation and ownership, thus offer global life science researchers with the highest quality products with an affordable pricing policy.

DyLight 405, Goat Anti-Mouse IgG Review

The antibodies marked by Dylight 405 is brighter than those marked by Cascade Blue. They are similar in brightness to those marked by Alexa Fluor 405,while having a lower background brightness. As for optical stability, both Dylight 405 and Alexa 405 perform well, better than Cascade Blue. In addition, the second antibody marked by Dylight have higher performance-price ratio than those marked by traditional fluorescence and Alexa.

Affinity purified using solid phase DyLight 405, Goat Anti-Mouse IgG with finally > 95% purity based on SDS-PAGE. And Abbkine suggested starting 1:50-1:1,000 dilutions for most fluorescent applications.

Dylight 405 is involved in some advantages, such as strong fluorescence, good optical stability, high specificity, good water-solubility, low background brightness and high performance-price ratio. Abbkine insists on offering the high quality products and perfect services to the masses of customers. It is right to choose Abbkine！

Cleaved-Caspase-3 p12 (D175) Polyclonal Antibody Released

Caspase 3, also known as CPP32, Yama, apopain, plays a key effectors of apoptosis. It acts downstream of caspase 9 in the apoptotic pathway. During apoptosis, it gets activated by proteolytic cleavage into the 17-19 kDa (p17, p18) and 12 kDa (p12) active subunits. Any alteraion in CASP-3 in mice, causes premature lethality. It has also shown that the deletion of gene affects brain development resulting of hyperplasias and disorganized cell deployement. Clinically it is related to Huntington disease (HD).

Abbkine Cleaved-Caspase-3 p12 (D175) Polyclonal Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using synthesized peptide derived from the internal region of human Caspase-3 p12 at AA rangle: 130-210. The Caspase-3 p12 antibody was tested to  react with human sample and can be applied in WB, IHC-P, ELISA. Abbkine suggested the starting dilutions are as follows: WB: 1:500-1:2000, IHC-p: 1:100-1:300, ELISA: 1:20000.

Cleaved-Caspase-3 p12 (D175) Polyclonal Antibody is supplied as liquid solution. The concentration is 1mg/ml. The antibody is stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Attention: the product is for research use only and is not intended for use in human or clinical diagnosis.

Cleaved-Caspase-1 (D210) Polyclonal Antibody Released

CASP1 (caspase 1) encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. CASP1 has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms.

Abbkine Cleaved-Caspase-1 (D210) Polyclonal Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using synthesized peptide derived from the internal region of human caspase-1. at AA rangle: 130-210. The antibody was tested to  react with human sample and can be applied in WB, IHC-P, ELISA. Abbkine suggested the starting dilutions are as follows: WB: 1:500-1:2000, IHC-p: 1:100-1:300, ELISA: 1:10000.

Cleaved-Caspase-1 (D210) Polyclonal Antibody is supplied as liquid solution. The concentration is 1mg/ml. The antibody is stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Attention: the product is for research use only and is not intended for use in human or clinical diagnosis.

DyLight 350, Goat Anti-Mouse IgG Review

To locate three different kinds of proteins in a sample through immunofluorescence technique, three different kinds of fluorophores,rather than pigments such as DAPI, are needed.( Namely, a green fluorescently-labeled secondary antibody、a red fluorescently-labeled secondary antibody，and a blue fluorescently-labeled secondary antibody). Dylight 350 blue fluorescence,the antibody offered by Abbkine, has higher intensity of fluorescence, better sensibility and higher optical stability than AMCA blue fluorescence.

Affinity purified using solid phase DyLight 350, Goat Anti-Mouse IgG with finally > 95% purity based on SDS-PAGE. And Abbkine suggested starting 1:50-1:1,000 dilutions for most fluorescent applications.

As the signals of AMCA are relatively weaker, AMCA is not recommended in single immunofluorescence analysis. Dylight 350, as excellent substitute for AMCA, has many advantages. I chose this product. I am quite satisfied.

DyLight 405, Goat Anti-Mouse IgG Review

The antibodies marked by Dylight 405 is brighter than those marked by Cascade Blue.They are similar in brightness to those marked by Alexa Fluor 405,while having a lower background brightness. As for optical stability, both Dylight 405 and Alexa 405 perform well, better than Cascade Blue. In addition, the secondary antibody marked  by Dylight have higher performance-price ratio than those marked by traditional fluorescence and Alexa.

Affinity purified using solid phase DyLight 405, Goat Anti-Mouse IgG with finally > 95% purity based on SDS-PAGE. And Abbkine suggested starting 1:50-1:1,000 dilutions for most fluorescent applications.

Dylight 405 is involved in some advantages, such as strong fluorescence, good optical stability, high specificity, good water-solubility, low background brightness and high performance-price ratio. Abbkine insists on offering the high quality products and perfect services to the masses of customers. It is right to choose Abbkine！

PurKine™ Benzamidine Resin 4FF Released

Benzamidine is a reversible competitive inhibitor of trypsin, trypsin-like enzymes and serine proteases. It is often used as a ligand in protein crystallography to prevent proteases from degrading a protein of interest.

Abbkine PurKine™ Benzamidine Resin 4FF consists of 90μm beads of highly cross-linked 4% agarose, to which aminobenzamidine has been coupled. It has been designed for one-step removal and/or affinity purification of trypsin, trypsin-like serine proteases, and zymogens including urokinase and prekallikrein from serum, monoclonal cell supernatants and bacterial lysates. Thrombin and factor Xa after tag cleavage of recombinant proteins have also been effectively removed by this resin.

Tests confirm that more than 20mg trypsin can be captured by one milliliter PurKine™ Benzamidine Resin 4FF and no decrease in performance occurs after at least five repeated uses. The highly cross-linked resin is capable of enduring linear flow rates of up to 300 centimeters per hour, which is excellent for scaling-up.

DyLight 488, Goat Anti-Mouse IgG Review

Abbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Affinity purified using solid phase DyLight 488, Goat Anti-Mouse IgG  with finally > 95% purity based on SDS-PAGE. And Abbkine suggested starting 1:50-1:1,000 dilutions for most fluorescent applications.

DyLight 488 conjugates fade less than FITC and Cy2 conjugates in mounting media indicating that the DyLight 488 molecule is inherently more photostable in epifluorescence microscopes. DyLight 488, Goat Anti-Mouse IgG has higher cost performance than traditional FITC/Cy2 and Alexa 488.

Abbkine Scientific Introduces New Heparin Resin 6FF Product

United States, 4^th Jan 2017 – Abbkine Scientific unravels another product under the Purification tool Category called Purkine Heparin Resin 6FF which is said to be effective in purifying a variety of heparin-binding proteins. Heparin is an anti-clotting agent found in the human body that can be used for the treatment of thrombosis or blood clots. Some of these proteins include Lipoprotein (proteins that carry lipids in the bloodstream); Anti-thrombin III (non-vitamin, K-dependent protease); and, proteins like transcription factors or virus coat that connect to Deoxyribonucleic Acid (DNA) molecules used in the growth and reproduction of living organisms.



This Purkine Heparin Resin 6FF is made up of 90μm beads (polystyrene-based microspheres) chemical bond six percent Agarose (polymer material) linked to Heparin. This facilitates optimization that leads to maximum yield of protein, capability to get dissolved and stability. There is no decline in performance even after five consecutive uses base on tests. The product is available in pre-packed kit formats and spin columns. This packaging is ideal for sample purification as well as preparations.

Abbkine Scientific makes use of the most recent procedures designed for synthetics together with advanced techniques in resin development as well as maximization of accessories. Purkine tools are made available for samples from nucleic acids including DNA and RNA, anti-bodies and bio-molecule markers. The end results are enhanced productivity, shortened workflow and more cost-savings.

Purkine Heparin Resin 6FF is formulated using a liquid solution of 50 percent semi-liquid mixture in 20 percent ethanol liquid. It has multiple benefits like density of Ligands (more than 4 milligrams of heparin and resin); high-performance; affordability; and minimal loss of Heparin Ligand and pH stability range from four until 12. High performance also means excellent binding capability and high rate of flow. The cross-linked beads are capable of enduring linear flow rates of up to 400 centimeters per hour. The Heparin can be stored for a maximum of one year at two to eight degrees Celsius from the date of its shipment. However, the product must not be kept in a freezer. This particular product is meant for research purposes only. It should not be used in humans or for any clinical diagnosis.

Abbkine Scientific states that applications of their products do not mean recommendations for usage. This can mean violation of patents or licenses. The company will not claim responsibility for patent violations or other breaches that may arise in case the product is used.

About the Abbkine Scientific

Abbkine Scientific Company focuses on the development, production and marketing of products for disciplines related to life science. It guarantees premium quality at reasonable costs. The bio-chemicals, anti-bodies, assay kits, and recombinant proteins of Abbkine are employed in practically all scientific environments and applications. These are bio-technology, cell research, diagnosis of diseases, and pharmaceutical development. The company strives constantly to reduce research costs without compromising reliability and values. Moreover, it makes sure that products are accessible from research quantities up to huge volumes for development and production.

The company’s exact address is Building 1, Harbor of Technology Times, (#35 Optical Valley Road), Wuhan (Hubei) China 430074. Check out the company’s website at http://www.abbkine.com for more information. Abbkine Scientific espouses the principle of Quality First, Customer First and Best Service for all of its clients. Company management also looks forward that all details in this Press Release can help readers in their research work. They are free to give feedback to the company by contacting support@abbkine.com for additional queries.

Exclusive Ni-Super Resin Product for His-tag Protein Purification released

Abbkine Scientific unravels an exclusive Ni-Super Resin portfolio under the Purification tools category called PurKine™ His-Tag Ni-Super Resin which is said to be featured two prominent advantages compared to Ni-NTA and Ni-IDA, high tolerance and easy cleaning.

Expression and purification of recombinant protein is the foundation of studying protein structure, regulation and function. To simplify purification, affinity purification tags are often fused to a recombinant protein of interest.The most popular tag is the polyhistidine (6xHis) tag. Some proteins, like eukaryotic proteins may not exhibit proper protein activity or folding if expressed in E.coli . Cultured mammalian cells or insect cells might be better option. However, the low expression levels of recombinant proteins in those cells presents a challenge for their purification.

[caption id="attachment_34942" align="aligncenter" width="485"] Structure of Ni-IDA, Ni-NTA and Ni-Super shows Ni-Super has two prominent advantages compared to Ni-NTA and Ni-IDA with higher tolerance and easier cleaning.[/caption]

Abbkine PurKine™ Ni-Super Resin is designed mainly for capturing and purification of histidine-tagged proteins secreted into eukaryotic cell, such as yeast, mammalian, and insect culture supernatants. PurKine™ Ni-Super Resin is a new immobilized metal ion affinity chromatography (IMAC) medium precharged with nickel ions via a proprietary, chemical-stable linker technology. The Resin consists of 90um beads of highly cross-linked 4% agarose, to which Super replacement to NTA has been coupled. Compared to Ni-NTA and Ni-IDA, Ni-Super resin has prominent advantages.

• High toleration— More tolerable to various chemicals including reducing, chelating agents. The nickel ions have been shown to remain bound to the chelating ligand even after incubation for 24 hours in 10 mM EDTA.


• Low nickel ion leaching— Best option for samples that can cause Ni stripping from the medium, like secreted proteins in liquids containing chelators. Do not need repeated regeneration.


• Time-saving sample preparation— Direct loading of samples without extra pre-treatment procedures, enabling purification of low concentrations of target proteins at large volumes.


• Easy cleaning— Directly use NaOH to cleaning-in-place, reducing cleaning cycle.

Ni-Super resin is more tolerable to various chemicals including reducing, chelating agents. The nickel ions have been shown to remain bound to the chelating ligand even after incubation for 24 hours with 10 mM EDTA. When cleaning, directly use NaOH. It is easy to pack and use, and its fast flow properties make it excellent for scaling-up. 



PurKine™ His-Tag Purification system is based on innovative high-capacity IMAC matrix for convenient single-step purifications of His-tag proteins from total lysates. Our propriety ion-chelate chemistry ensures extraordinary compatibility with commonly used reducing agents such as DTT, chelating metalloprotease inhibitors such as EDTA, and a wide range of buffer substances and salt conditions. The portfolio provides wide choice of chelating group (IDA, NTA or Super NTA), cross-linked agarose (crosslinked 4% or 6% agarose), ions (Nick, Colbat, Copper, or non ion charged) and compatible ingredients to allow optimization of process for maximum protein yield, stability and solubility. Tests also confirm that no decrease in performance occurs after at least five repeated uses. The PurKine™ His-Tag Purification resin is also available as prepacked spin column and kit formats.

Abbkine Scientific Co, Ltd was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Abbkine started featured and exclusive products from protein detection tools, and devoted to developing and providing the most innovative solutions for global customers covering all kinds of antibodies, biochemicals, proteins and assay kits.