AFP alpha 1 Fetoprotein Monoclonal Antibody Review

Alpha-fetoprotein (AFP, α-fetoprotein; also sometimes called alpha-1-fetoprotein, alpha-fetoglobulin, or alpha fetal protein) is a protein that in humans is encoded by the AFP gene. The AFP gene is located on the q arm of chromosome 4 (4q25). AFP is a major plasma protein produced by the yolk sac and the liver during fetal development. It is thought to be the fetal form of serum albumin. AFP binds to copper, nickel, fatty acids and bilirubin and is found in monomeric, dimeric and trimeric forms.

Abbkine AFP alpha 1 Fetoprotein Monoclonal Antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. The antibody reacts with human alpha-Fetoprotein (AFP) and was validated for WB, IF, IHC-p. Abbkine suggested the starting dilutions for WB was 1:2000, IF: 1:200, IHC-p: 1:200, but the optimal working dilutions should be determined experimentally by the end user. The concentration of the antibody is 1mg/ml.

Abbkine AFP alpha 1 Fetoprotein Monoclonal Antibody was used in Immunofluorescence analysis of human breast cancer tissue. It was diluted at 1:200 (4°C, overnight). IFKine red  anti-mouse IgG secondary antibody was diluted at 1:300 (room temperature, 50min). There was a stronger IF signal and the signal is localizing where I expect it to. I give it a high recommendation and plan to use it again.