ERK1 Mouse Monoclonal Antibody (5E9) Review

MAPK1 encodes a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for MAPK1.

Abbkine ERK1 Mouse Monoclonal Antibody (5E9) was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. The concentration of this antibody is 1mg/ml. This antibody could be applied to IF and IHC-p experiments. It can react with human, rat and mouse samples. Optimal working dilutions should be determined experimentally by the investigator. The suggested starting dilution for IHC-p is 1:100-200.

As for our new project, we need to detect the endogenous ERK1 protein in human uterus tissue. I conducted the Immunohistochemistry experiments, using the Abbkine ERK1 Mouse Monoclonal Antibody. The conditions are as follows: ERK1 Mouse Monoclonal Antibody (5E9) was diluted at 1:200 (4°C, overnight). Finally, the results are amazing and my mentor was very satisfied with this.  I have to say that Abbkine antibody has high specificity and strong stability. Recently, I want to recommend it to one of my friends in The University of Manchester.