Cytokeratin 6 Monoclonal Antibody review

Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue, where they constitute up to 85% of mature keratinocytes in the vertebrate epidermis. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. The alpha-helical coiled-coil dimers associate laterally end-to-end to form 10 nm diameter filaments. Cytokeratins, which are useful markers of tissue differentiation, also aid in the characterization of malignant tumors. IL-1 and TNFα induce transcription of cytokeratin 6 in epidermal keratinocytes via the C/EBP β transcription factor. In humans, multiple isoforms of cytokeratin 6 (6A-6F), encoded by several highly homologous genes, have distinct tissue expression patterns, and cytokeratin 6A is the dominant form in epithelial tissue. The gene encoding human cytokeratin 6A maps to chromosome 12q13, and mutations in this gene are linked to several inheritable hair and skin pathologies.

Abbkine Cytokeratin 6 Monoclonal Antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. The antibody detects endogenous Cytokeratin 6 proteins. It was validated for WB, IHC-p, IF and tested in Human. The original concentration of this antibody is 1mg/ml. During the experiment, optimal working dilutions should be explored by the researchers. The suggested starting dilution for WB is 1:2000;  IF/IHC-p 1: 200.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue labelling Cytokeratin 6 with purified Abbkine Cytokeratin 6 Monoclonal Antibody at 1/200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A HRP-conjugated goat anti-mouse IgG (H+L) was used as the secondary antibody. Negative control using PBS instead of primary antibody. The signal produced is strong and the results are accord with my experiment expectation.