2016年12月8日星期四

Affinity of Protein A, Protein G and Protein A/G

Protein A, Protein G and Protein A/G are often used in antibody purification and other applications such as immunoprecipitation (IP) and chromatin immunoprecipitation(ChIP). Their binding abilities to antibodies of different subtypes and species are varied. How to choose correct types of protein to purify your sample? Here, we list the antibody binding properties of Protein A, Protein G and Protein A/G.

Note: +++ strong binding; ++ medium binding; + weak binding; - no binding













































































































































SpeciesAntibody subtypeProtein AProtein GProtein A/G
HumanTotal IgG+++++++++
IgG1+++++++++
IgG2+++++++++
IgG3+++++++
IgG4+++++++++
IgM+-+
IgD---
IgA+-+
MouseTotal IgG+++++++++
IgG2a+++++++++
IgG2b++++++
IgG3+++++
IgM---
RatTotal IgG+++++
 IgG1+++++
 IgG2a-++++++
 IgG2b-++
 IgG2c+++++++++
RabbitTotal IgG+++++++++
GoatTotal IgG+++++++
SheepTotal IgG+++++++
Guinea pigTotal IgG+++++++
HamsterTotal IgG++++++
DonkeyTotal IgG++++++++
HorseTotal IgG+++++++
CowTotal IgG+++++++
PigTotal IgG+++++++
DogTotal IgG+++++++
CatTotal IgG+++++++
ChickenTotal IgY---

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