VSV-G Tag Sequence

VSV-G, a vesicular stomatitis virus G (VSV-G) protein fragment, which is commonly used in biomedical research to pseudotype retroviral and lentiviral vectors, conveying the ability to transduce a broad stroll of mammalian cell sign with genes of interest.

As show below, recognized VSV-G epitope represents the amino acid sequence derived from the Vesicular Stomatitis viral glycoprotein.



 

The VSV-G epitope tag is commonly engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods.

VSV-G Tag DNA Sequence

We cited VSV-G sequence information from Plasmid pCMV-VSV-G, which is from Dr. Bob Weinberg's lab published in RNA 2003 Apr;9(4):493-501. In this vector, VSV-G tag sequence coding envelope protein for producing lentiviral and MuLV retroviral particles has been used in conjunction with a packaging vector such as pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral).



DNA sequence coding for VSVG tag in pCMV-VSV-G vector is from 2936-2968 with total 33 nucleotides, TAT ACA GAC ATA GAG ATG AAC CGA CTT GGA AAG.

VSV-G Tag Amino Acid Sequence

VSV-G epitope tag is corresponding aa 501-511 of vesicular stomatitis virus glycoprotein (VSV-G), which is a 11 amino acid sequence YTDIEMNRLGK peptide derived from the Vesicular Stomatitis viral glycoprotein.

Sequence	Tyr-Thr-Asp-Ile-Glu-Met-Asn-Arg-Leu-Gly-Lys
Chemical Name	VSV-G Peptide
Canonical SMILES	CCC(C)C(C(=O)NC(CCC(=O)O)C(=O)NC(CCSC)C (=O)NC(CC(=O)N)C(=O)NC(CCCN=C(N)N)C(=O) NC(CC(C)C)C(=O)NCC(=O)NC(CCCCN)C(=O)O)N C(=O)C(CC(=O)O)NC(=O)C(C(C)O)NC(=O)C(CC1 =CC=C(C=C1)O)N
Formula	C57H94N16O19S	M.Wt	1334

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