2016年12月22日星期四

Anti-TAP Tag Monoclonal Antibody(4H2) Review

Tandem affinity purification (TAP) is a purification technique for studying protein–protein interactions. It involves creating a fusion protein with a designed piece, the TAP tag, on the end.The TAP tag historically consists of a calmodulin binding peptide (CPB), a tobacco etch virus (TEV) protease cleavage site, and Protein A. However, additional tag combinations have been used with the TAP a02200-wbmethod including the combination of FLAG tags and HA tags.The TAP method permits the identification of proteins interacting with a particular target protein without any prior knowledge about the function, activity, or composition of the interacting proteins. This tag is also known as the C-terminal TAP tag because an N-terminal version is also available. However, the method to be described assumes the use of a C-terminal tag, although the principle behind the method is still the same.

Anti-TAP Tag Monoclonal Antibody(4H2) was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This antibody has been tested with WB. And Abbkine suggested starting dilutions are as follows: WB 1:5000-10000.

The TAP (Tandem Affinity Purification) method is an affinity purification method for the isolation of TAP-tagged proteins along with associated proteins. To my knowledge, Abbkine is a great option when you want to find a cost-effective product. It isworth recommending.

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