How to Choose good Amcyan antibody

One of the most frustrating things in the lab is spending good money on an antibody only to find out it doesn’t work well in your application. You can then either spend weeks trying to make it work, abandon your work altogether or humbly ‘fess up that you made a bad purchase and beg for a second chance. In an effort to limit your groveling, we have made a list of tips for choosing a good Amcyan antibody for Amcyan epitope or Amcyan fusion proteins.

Pay attention to application

The best case scenario is to find a Cyman tag antibody that has already been used in the exact manner in which you intend to use it. Most of us are rarely this lucky!

Search the literature, product reviews and product data sheets to determine how the antibody has been used in the past. Be mindful that antibodies that work well in one application may not be suited for another. For example, an antibody that works well in a Western may not work well in immunoprecipitations due to conformational changes in the protein. Alternatively, posttranslational modifications, such as phosphorylation, might mask or reveal epitopes recognized by antibodies (particularly monoclonal antibodies).

If you can’t find an antibody that has been used in your application, choose one that has been used in a compatible manner. For example, antibodies used in FACS analysis on live cells might also work in native immunoprecipitations which also maintain the integrity of the protein.

Check how the Amcyan antibody was validated

A lot of antibodies are validated using recombinant proteins that are abundantly expressed. Or they are validated with proteins that were produced in non-native hosts such as bacteria or yeast. While these validations show that the antibody can work, they do not necessarily show that the antibody will work under alternative conditions.

[caption id="attachment_18" align="aligncenter" width="120"] Western blot analysis of AmCyan fusion protein with anti-AmCyan tag monoclonal antibody(8T2) at 1:5000 (lane A) dilution.[/caption]

The type of antibody matters

Is it better to us a monoclonal or a polyclonal Amcyan antibody? For some proteins, you don’t have a say in the matter – you have to go with whatever antibody is available. However, if you do have a choice, choosing the right kind of antibody might increase your success. Polyclonal antibodies, which recognize multiple epitopes, are often better in Western blots, immunohistochemistry and immunoprecipitations. They can also be more robust and detect less abundant proteins.

Monoclonals are useful in detecting conformation-specific epitopes and are invaluable when using multiple antibodies simultaneously. Due to their specificity, monoclonals often give less background. Because they only recognize a single epitope though, monoclonal antibodies can be more limited in application. But don’t let that scare you off! I have used many monoclonal anbtibodies that worked well in Westerns.

If you are looking for a detection tool to quantify or localize your Amcyan epitope or Amcyan fusion proteins, we highly recommend you to try our featured monoclonal Amcyan Antibody risk free. This antibody with clone number 8T2 can specifically recognizes native and denatured forms of Amcyan fusion proteins, with which can meet your any requirements for Western Blot analysis.